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PMID: 11867538 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Hyperacetylation of chromatin at the ADH2 promoter allows Adr1 to bind in repressed conditions.

The EMBO journal ·Vol. 21 ·No. 5 ·2002-03-01 ·Pages 1101-11

Verdone L, Wu J, van Riper K, Kacherovsky N, Vogelauer M, Young ET, Grunstein M, Di Mauro E, Caserta M

Abstract

We report that in vivo increased acetylation of the repressed Saccharomyces cerevisiae ADH2 promoter chromatin, as obtained by disrupting the genes for the two deacetylases HDA1 and RPD3, destabilizes the structure of the TATA box-containing nucleosome. This acetylation-dependent chromatin remodeling is not sufficient to allow the binding of the TATA box-binding protein, but facilitates the recruitment of the transcriptional activator Adr1 and induces faster kinetics of mRNA accumulation when the cells are shifted to derepressing conditions.

MeSH Terms
Acetylation Acetyltransferases/deficiency,genetics,metabolism Alcohol Dehydrogenase/genetics Chromatin/chemistry,genetics,metabolism DNA-Binding Proteins/metabolism Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Histone Acetyltransferases Histone Deacetylases/deficiency,genetics,metabolism Histones/metabolism Models, Genetic Nucleosomes/chemistry,metabolism,ultrastructure Promoter Regions, Genetic/genetics Protein Kinases/deficiency,genetics,metabolism Protein Processing, Post-Translational/genetics RNA, Messenger/biosynthesis Repressor Proteins Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism TATA Box/genetics TATA-Box Binding Protein Transcription Factors/metabolism Transcriptional Activation
Chemicals
ADR1 protein, S cerevisiae Chromatin DNA-Binding Proteins Fungal Proteins Histones Nucleosomes RNA, Messenger Repressor Proteins Saccharomyces cerevisiae Proteins TATA-Box Binding Protein Transcription Factors Alcohol Dehydrogenase Acetyltransferases Histone Acetyltransferases Protein Kinases HDA1 protein, S cerevisiae RPD3 protein, S cerevisiae Histone Deacetylases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Verdone Loredana
Fondazione Istituto Pasteur-Fondazione Cenci Bolognetti and Centro di Studio per gli Acidi Nucleici, CNR, c/o Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, I-00185 Rome, Italy.
Wu Jiansheng
van Riper Kristen
Kacherovsky Nataly
Vogelauer Maria
Young Elton T
Grunstein Michael
Di Mauro Ernesto
Caserta Micaela
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/21.5.1101
Published
2002-03-01
Pages
1101-11
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125900
Subset
IM
Grants
NIGMS NIH HHS · GM 23674 · United States
NIGMS NIH HHS · R01 GM023674 · United States
NIGMS NIH HHS · GM 26079 · United States
NIGMS NIH HHS · R01 GM026079 · United States
NIGMS NIH HHS · R37 GM023674 · United States
External Links
PubMed source DOI: 10.1093/emboj/21.5.1101 PMC Full Text View journal details
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