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PMID: 11940677 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heterochromatin protein 1 is involved in control of telomere elongation in Drosophila melanogaster.

Molecular and cellular biology ·Vol. 22 ·No. 9 ·2002-05-00 ·Pages 3204-18

Savitsky M, Kravchuk O, Melnikova L, Georgiev P

Abstract

Telomeres of Drosophila melanogaster contain arrays of the retrotransposon-like elements HeT-A and TART. Their transposition to broken chromosome ends has been implicated in chromosome healing and telomere elongation. We have developed a genetic system which enables the determination of the frequency of telomere elongation events and their mechanism. The frequency differs among lines with different genotypes, suggesting that several genes are in control. Here we show that the Su(var)2-5 gene encoding heterochromatin protein 1 (HP1) is involved in regulation of telomere length. Different Su(var)2-5 mutations in the heterozygous state increase the frequency of HeT-A and TART attachment to the broken chromosome end by more than a hundred times. The attachment occurs through either HeT-A/TART transposition or recombination with other telomeres. Terminal DNA elongation by gene conversion is greatly enhanced by Su(var)2-5 mutations only if the template for DNA synthesis is on the same chromosome but not on the homologous chromosome. The Drosophila lines bearing the Su(var)2-5 mutations maintain extremely long telomeres consisting of HeT-A and TART for many generations. Thus, HP1 plays an important role in the control of telomere elongation in D. melanogaster.

MeSH Terms
Animals Base Sequence Chromosomal Proteins, Non-Histone/genetics,metabolism Chromosome Aberrations Crosses, Genetic DNA/genetics,metabolism DNA Transposable Elements Drosophila Proteins/genetics,metabolism Drosophila melanogaster/genetics Female Gene Conversion Gene Duplication Gene Expression Regulation Gene Products, gag Genes, Insect/genetics Insect Proteins/genetics,metabolism Male Mutation Physical Chromosome Mapping Telomere/genetics,metabolism Templates, Genetic
Chemicals
Chromosomal Proteins, Non-Histone DNA Transposable Elements Drosophila Proteins Gene Products, gag HeT-A-gag protein, Drosophila Insect Proteins heterochromatin protein 1, Drosophila DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Savitsky Mikhail
Department of Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 117334 Moscow, Russia.
Kravchuk Oksana
Melnikova Larisa
Georgiev Pavel
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.22.9.3204-3218.2002
Published
2002-05-00
Pages
3204-18
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC133762
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.22.9.3204-3218.2002 PMC Full Text View journal details
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