Home LiteratureArticle Details
PMID: 12234854 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A resistance-nodulation-cell division family xenobiotic efflux pump in an obligate anaerobe, Porphyromonas gingivalis.

Antimicrobial agents and chemotherapy ·Vol. 46 ·No. 10 ·2002-10-00 ·Pages 3257-60

Ikeda T, Yoshimura F

Abstract

Porphyromonas gingivalis, a gram-negative obligate anaerobe, contains two homologs of an Escherichia coli resistance-nodulation-cell division-type multidrug exporter gene, acrB, in putative operons, together with homologs of membrane fusion protein gene acrA and outer membrane channel gene tolC. MIC determination and accumulation assays with mutants with disruptions of one or more genes showed that one cluster, named xepCAB, pumped out multiple agents including rifampin, puromycin, and ethidium bromide.

MeSH Terms
Anaerobiosis Anti-Bacterial Agents/metabolism,pharmacology Bacterial Proteins/genetics,metabolism Carrier Proteins/genetics,metabolism Drug Resistance, Multiple, Bacterial Escherichia coli/genetics,metabolism Escherichia coli Proteins Membrane Proteins Microbial Sensitivity Tests Multidrug Resistance-Associated Proteins Multigene Family Porphyromonas gingivalis/drug effects,metabolism Xenobiotics/metabolism,pharmacology
Chemicals
AcrB protein, E coli Anti-Bacterial Agents Bacterial Proteins Carrier Proteins Escherichia coli Proteins Membrane Proteins Multidrug Resistance-Associated Proteins Xenobiotics
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ikeda Takeshi
Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya 464-8650, Japan. [email protected]
Yoshimura Fuminobu
References (20)
20 references, click to expand
  1. AcrD of Escherichia coli is an aminoglycoside efflux pump.
    J Bacteriol. 2000 Mar;182(6):1754-6 PMID: 10692383
  2. Transcriptional organization of the czc heavy-metal homeostasis determinant from Alcaligenes eutrophus.
    J Bacteriol. 1999 Apr;181(8):2385-93 PMID: 10198000
  3. Accumulation of norfloxacin by Bacteroides fragilis.
    Antimicrob Agents Chemother. 2000 Sep;44(9):2361-6 PMID: 10952580
  4. Molecular properties of bacterial multidrug transporters.
    Microbiol Mol Biol Rev. 2000 Dec;64(4):672-93 PMID: 11104814
  5. Genes involved in copper homeostasis in Escherichia coli.
    J Bacteriol. 2001 Mar;183(6):2145-7 PMID: 11222619
  6. Analysis of a complete library of putative drug transporter genes in Escherichia coli.
    J Bacteriol. 2001 Oct;183(20):5803-12 PMID: 11566977
  7. Signal sequences. The limits of variation.
    J Mol Biol. 1985 Jul 5;184(1):99-105 PMID: 4032478
  8. Lipoproteins in bacteria.
    J Bioenerg Biomembr. 1990 Jun;22(3):451-71 PMID: 2202727
  9. Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways.
    Gene. 1992 Nov 2;121(1):47-54 PMID: 1427098
  10. Molecular cloning and characterization of acrA and acrE genes of Escherichia coli.
    J Bacteriol. 1993 Oct;175(19):6299-313 PMID: 8407802
  11. A family of extracytoplasmic proteins that allow transport of large molecules across the outer membranes of gram-negative bacteria.
    J Bacteriol. 1994 Jul;176(13):3825-31 PMID: 8021163
  12. Two novel families of bacterial membrane proteins concerned with nodulation, cell division and transport.
    Mol Microbiol. 1994 Mar;11(5):841-7 PMID: 8022262
  13. Molecular and genetic analysis of the Bacteroides uniformis cephalosporinase gene, cblA, encoding the species-specific beta-lactamase.
    Antimicrob Agents Chemother. 1994 Aug;38(8):1711-5 PMID: 7985999
  14. Virulence of a Porphyromonas gingivalis W83 mutant defective in the prtH gene.
    Infect Immun. 1995 Apr;63(4):1521-8 PMID: 7890419
  15. Multidrug efflux pumps of gram-negative bacteria.
    J Bacteriol. 1996 Oct;178(20):5853-9 PMID: 8830678
  16. Proton-dependent multidrug efflux systems.
    Microbiol Rev. 1996 Dec;60(4):575-608 PMID: 8987357
  17. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
    Nucleic Acids Res. 1997 Sep 1;25(17):3389-402 PMID: 9254694
  18. Systemic antibiotic therapy in periodontics.
    Periodontol 2000. 1996 Feb;10:45-78 PMID: 9567937
  19. Active efflux of norfloxacin by Bacteroides fragilis.
    Antimicrob Agents Chemother. 1998 Aug;42(8):2119-21 PMID: 9687419
  20. Primer for antimicrobial periodontal therapy.
    J Periodontal Res. 2000 Apr;35(2):108-14 PMID: 10863965
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.46.10.3257-3260.2002
Email
[email protected]
Published
2002-10-00
Pages
3257-60
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC128772
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.46.10.3257-3260.2002 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2