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PMID: 12374737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cdc37 is essential for chromosome segregation and cytokinesis in higher eukaryotes.

The EMBO journal ·Vol. 21 ·No. 20 ·2002-10-15 ·Pages 5364-74

Lange BM, Rebollo E, Herold A, González C

Abstract

Cdc37 has been shown to be required for the activity and stability of protein kinases that regulate different stages of cell cycle progression. However, little is known so far regarding interactions of Cdc37 with kinases that play a role in cell division. Here we show that the loss of function of Cdc37 in Drosophila leads to defects in mitosis and male meiosis, and that these phenotypes closely resemble those brought about by the inactivation of Aurora B. We provide evidence that Aurora B interacts with and requires the Cdc37/Hsp90 complex for its stability. We conclude that the Cdc37/Hsp90 complex modulates the function of Aurora B and that most of the phenotypes brought about by the loss of Cdc37 function can be explained by the inactivation of this kinase. These observations substantiate the role of Cdc37 as an upstream regulatory element of key cell cycle kinases.

MeSH Terms
Animals Aurora Kinases Cell Cycle Cell Cycle Proteins/genetics,metabolism Cell Division Chromosome Segregation Drosophila/cytology,genetics,metabolism Drosophila Proteins/genetics,metabolism Genes, Insect HSP90 Heat-Shock Proteins/metabolism Male Meiosis Molecular Chaperones/genetics,metabolism Mutation Protein Serine-Threonine Kinases/metabolism RNA Interference Spermatocytes/cytology,metabolism Spindle Apparatus/metabolism
Chemicals
Cdc37 protein, Drosophila Cell Cycle Proteins Drosophila Proteins HSP90 Heat-Shock Proteins Molecular Chaperones Aurora Kinases Protein Serine-Threonine Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lange Bodo M H
European Molecular Biology Laboratory, Cell Biology and Biophysics Programme and Gene Expression Programme, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. [email protected]
Rebollo Elena
Herold Andrea
González Cayetano
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdf531
Email
[email protected]
Published
2002-10-15
Pages
5364-74
Language
English
Region
England
NLM ID
8208664
PMCID
PMC129064
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdf531 PMC Full Text View journal details
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