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PMID: 12839759 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification and characterization of a GDSL esterase gene located proximal to the swr quorum-sensing system of Serratia liquefaciens MG1.

Applied and environmental microbiology ·Vol. 69 ·No. 7 ·2003-07-00 ·Pages 3901-10

Riedel K, Talker-Huiber D, Givskov M, Schwab H, Eberl L

Abstract

Serratia liquefaciens MG1 employs the swr quorum-sensing system to control various functions, including production of extracellular enzymes and swarming motility. Here we report the sequencing of the swr flanking DNA regions. We identified a gene upstream of swrR and transcribed in the same direction, designated estA, which encodes an esterase that belongs to family II of lipolytic enzymes. EstA was heterologously expressed in Escherichia coli, and the substrate specificity of the enzyme was determined in crude extracts. With the aid of zymograms visualizing EstA on polyacrylamide gels and by the analysis of a transcriptional fusion of the estA promoter to the promoterless luxAB genes, we showed that expression of the esterase is not regulated by the swr quorum-sensing system. An estA mutant was generated and was found to exhibit growth defects on minimal medium containing Tween 20 or Tween 80 as the sole carbon source. Moreover, we show that the mutant produces greatly reduced amounts of N-acyl-homoserine lactone (AHL) signal molecules on Tween-containing medium compared with the wild type, suggesting that under certain growth conditions EstA may be important for providing the cell with precursors required for AHL biosynthesis.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Escherichia coli/enzymology,genetics Esterases/chemistry,genetics,metabolism Gene Expression Regulation, Bacterial Molecular Sequence Data Sequence Alignment Sequence Analysis, DNA Serratia/enzymology,genetics,growth & development,physiology Signal Transduction Substrate Specificity
Chemicals
Bacterial Proteins Esterases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Riedel Kathrin
Department of Microbiology, Technical University Munich, Am Hochanger 4, D-85350 Freising, Germany.
Talker-Huiber Daniela
Givskov Michael
Schwab Helmut
Eberl Leo
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.69.7.3901-3910.2003
Published
2003-07-00
Pages
3901-10
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC165140
Subset
IM
Databases
GENBANK
AY168877
External Links
PubMed source DOI: 10.1128/AEM.69.7.3901-3910.2003 PMC Full Text View journal details
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