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PMID: 12970487 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Members of the Arabidopsis-SKP1-like gene family exhibit a variety of expression patterns and may play diverse roles in Arabidopsis.

Plant physiology ·Vol. 133 ·No. 1 ·2003-09-00 ·Pages 203-17

Zhao D, Ni W, Feng B, Han T, Petrasek MG, Ma H

Abstract

Ubiquitin-mediated proteolysis by the proteasome is a critical regulatory mechanism controlling many biological processes. In particular, SKP1, cullin/CDC53, F-box protein (SCF) complexes play important roles in selecting substrates for proteolysis by facilitating the ligation of ubiquitin to specific proteins. In plants, SCF complexes have been found to regulate auxin responses and jasmonate signaling and may be involved in several other processes, such as flower development, circadian clock, and gibberellin signaling. Although 21 Skp1-related genes, called Arabidopsis-SKP1-like (ASK), have been uncovered in the Arabidopsis genome, ASK1 is the only gene that has been analyzed genetically. As a first step toward understanding their functions, we tested for expression of 20 ASK genes using reverse transcription-polymerase chain reaction experiments. Also, we examined the expression patterns of 11 ASK genes by in situ hybridizations. The ASK genes exhibit a spectrum of expression levels and patterns, with a large subset showing expression in the flower and/or fruit. In addition, the ASK genes that have similar sequences tend to have similar expression patterns. On the basis of the expression results, we selectively suppressed the expression of a few ASK genes using RNA interference. Compared with the ask1 mutant, the strong ASK1 RNA interference (RNAi) line exhibited similar or enhanced phenotypes in both vegetative and floral development, whereas ASK11 RNAi plants had normal vegetative growth but mild defects in flower development. The diverse expression patterns and distinct defects observed in RNAi plants suggest that the ASK gene family may collectively perform a range of functions and may regulate different developmental and physiological processes.

MeSH Terms
Arabidopsis/genetics,growth & development Arabidopsis Proteins/genetics,metabolism Flowers/genetics,growth & development Gene Expression Profiling/methods Gene Expression Regulation, Developmental Gene Expression Regulation, Plant In Situ Hybridization Multigene Family/genetics Phenotype Phylogeny RNA Interference/physiology Reproduction/genetics SKP Cullin F-Box Protein Ligases/genetics,metabolism
Chemicals
ASK1 protein, Arabidopsis Arabidopsis Proteins SKP Cullin F-Box Protein Ligases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zhao Dazhong
Department of Biology and Huck Institute for Life Sciences, 313 Wartik Laboratory, The Pennsylvania State University, University Park, PA 16802, USA.
Ni Weimin
Feng Baomin
Han Tianfu
Petrasek Megan G
Ma Hong
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.103.024703
Published
2003-09-00
Pages
203-17
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC196598
Subset
IM
Grants
NIGMS NIH HHS · R01 GM 63871 · United States
External Links
PubMed source DOI: 10.1104/pp.103.024703 PMC Full Text View journal details
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