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PMID: 1321285 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

trans-acting requirements for replication of Epstein-Barr virus ori-Lyt.

Journal of virology ·Vol. 66 ·No. 8 ·1992-08-00 ·Pages 5030-9

Fixman ED, Hayward GS, Hayward SD

Abstract

Epstein-Barr virus (EBV) utilizes a completely different mode of DNA replication during the lytic cycle than that employed during latency. The latency origin of replication, ori-P, which functions in the replication of the latent episomal form of the EBV genome, requires only a single virally encoded protein, EBNA-1, for its activity. During the lytic cycle, a separate origin, ori-Lyt, is utilized. Relatively little is known about the trans-acting proteins involved in ori-Lyt replication. We established a cotransfection-replication assay to identify EBV genes whose products are required for replication of ori-Lyt. In this assay, a BamHI-H plasmid containing ori-Lyt was replicated in Vero cells cotransfected with the BamHI-H target, the three EBV lytic-cycle transactivators Zta, Rta, and Mta, and the EBV genome provided in the form of a set of six overlapping cosmid clones. By removing individual cosmids from the cotransfection mixture, we found that only three of the six cosmids were necessary for ori-Lyt replication. Subcloning of the essential cosmids led to the identification of six EBV genes that encode replication proteins. These genes and their functions (either known or predicted on the basis of sequence comparison with herpes simplex virus) are BALF5, the DNA polymerase; BALF2, the single-stranded DNA-binding protein homolog; BMRF1, the DNA polymerase processivity factor; BSLF1 and BBLF4, the primase and helicase homologs; and BBLF2/3, a potential homolog of the third component of the helicase-primase complex. In addition, ori-Lyt replication in this cotransfection assay was also dependent on one or more genes provided by the EBV SalI-F fragment and on the three lytic-cycle transactivators Zta, Rta, and Mta.

Related Genes
BALF2 BALF5 BBLF1 BBLF2 BBLF3 BMRF1 BSLF1
MeSH Terms
Amino Acid Sequence Animals Antigens, Viral/genetics,metabolism Base Sequence Cosmids DNA Primase DNA Replication DNA, Viral/genetics Epstein-Barr Virus Nuclear Antigens Genome, Viral Herpesvirus 4, Human/genetics,physiology Molecular Sequence Data Oligodeoxyribonucleotides Open Reading Frames Plasmids Polymerase Chain Reaction RNA Nucleotidyltransferases/genetics Restriction Mapping Sequence Homology, Nucleic Acid Simplexvirus/genetics Transcriptional Activation Transfection Vero Cells
Chemicals
Antigens, Viral DNA, Viral Epstein-Barr Virus Nuclear Antigens Oligodeoxyribonucleotides DNA Primase RNA Nucleotidyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fixman E D
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Hayward G S
Hayward S D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.8.5030-5039.1992
Published
1992-08-00
Pages
5030-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241360
Subset
IM
Grants
NCI NIH HHS · 5T32 CA09243 · United States
NCI NIH HHS · R01 CA22130 · United States
NCI NIH HHS · R37 CA30356 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.8.5030-5039.1992 PMC Full Text View journal details
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