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PMID: 1376370 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Constitutive expression of an ISGF2/IRF1 transgene leads to interferon-independent activation of interferon-inducible genes and resistance to virus infection.

Journal of virology ·Vol. 66 ·No. 7 ·1992-07-00 ·Pages 4470-8

Pine R

Abstract

Interferon (IFN)-stimulated gene factor 2 (ISGF2) plays a role in transcription of the beta IFN (IFN-beta) gene and IFN-stimulated genes (ISGs) and may function as a central mediator of cytokine responses. Constitutive ISGF2 transgene expression resulted in substantial resistance to three RNA virus families. This phenotype was not a consequence of IFN production and may have arisen directly through ISG expression. ISGF2 acted generally as a positive transcription factor through binding sites from several genes, in the context of transient cotransfection. Constitutive transcription of the endogenous IFN-beta gene, and several genes that are normally induced by either IFN-alpha or IFN-gamma, or only by IFN-alpha, was elevated in cells that constitutively express an ISGF2 transgene. However, constitutive and virus-induced levels of IFN-beta mRNA were unaffected in such cell lines.

MeSH Terms
Antibodies, Viral/immunology Base Sequence DNA, Viral DNA-Binding Proteins/genetics,physiology Gene Expression Regulation HeLa Cells Humans Immunity, Innate/genetics Interferon Regulatory Factor-1 Interferons/genetics,metabolism Molecular Sequence Data Neutralization Tests Phosphoproteins/genetics,physiology Transcription Factors/genetics,physiology Transfection Virus Diseases/immunology Virus Replication
Chemicals
Antibodies, Viral DNA, Viral DNA-Binding Proteins IRF1 protein, human Interferon Regulatory Factor-1 Phosphoproteins Transcription Factors Interferons
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Pine R
Laboratory of Molecular Cell Biology, Rockefeller University, New York, New York 10021-6399.
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.7.4470-4478.1992
Published
1992-07-00
Pages
4470-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241256
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.7.4470-4478.1992 PMC Full Text View journal details
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