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PMID: 6174973 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of interferon mRNA in human fibroblast cells induced to produce interferon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 12 ·1981-12-00 ·Pages 7426-30

Raj NB, Pitha PM

Abstract

The levels of interferon mRNA as a function of interferon induction by poly(rI) . poly(rC) in human fibroblast cells were determined by RNA hybridization using a cloned beta interferon cDNA and by translation in Xenopus oocytes. Whereas previous studies analyzed mixtures of interferons, the availability of the cloned beta interferon cDNA and the antiserum to purified beta interferon enabled us to focus on the expression of only one class (beta) of interferon genes. The induction of interferon synthesis depends primarily on the accumulation of interferon beta mRNA in the cells, and the interferon beta mRNA rapidly disappears several hours after its appearance in the cytoplasm. No detectable interferon beta mRNA sequences are present in uninduced cells. The degradation of interferon beta mRNA in the induced cells requires ongoing protein synthesis; accumulation of interferon beta mRNA was observed in the continuous presence of cycloheximide. The interferon beta mRNA detected at the early stages of induction is 1100 nucleotides long and its size progressively decreases with time. By both the hybridization and the translational assay in Xenopus oocytes, only one size of interferon beta mRNA and one species of beta interferon could be identified.

MeSH Terms
Cells, Cultured DNA/genetics Fibroblasts Humans Interferons/biosynthesis,genetics Molecular Weight Poly I-C/pharmacology RNA, Messenger/metabolism
Chemicals
RNA, Messenger DNA Interferons Poly I-C
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Raj N B
Pitha P M
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.12.7426
Published
1981-12-00
Pages
7426-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349280
Subset
IM
Grants
NIAID NIH HHS · 5R0I-AI-10944-09 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.12.7426 PMC Full Text View journal details
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