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PMID: 1385777 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

T cell receptor (TCR) beta chain homodimers on the surface of immature but not mature alpha, gamma, delta chain deficient T cell lines.

The EMBO journal ·Vol. 11 ·No. 7 ·1992-07-00 ·Pages 2735-45

Groettrup M, Baron A, Griffiths G, Palacios R, von Boehmer H

Abstract

Transfected T cell receptor (TCR) beta chain genes are expressed as homodimers on the surface of immature (Sci/ET27F) but not on mature (58 alpha-beta-) T cell lines which lack TCR alpha, gamma and delta chains. The homodimer on Sci/ET27F cells is tightly bound to CD3 delta and CD3 epsilon while the association with CD3 gamma and CD3 zeta proteins is rather weak. Crosslinking of the TCR beta homodimers resulted in a strong and rapid calcium flux. In 58 alpha-beta- T cells the beta TCR chain could be easily visualized intracellularly but was not transported to the cell surface. The Scid cell lines considerably facilitate the molecular analysis of early differentiation events in the thymus which are likely to be regulated by the beta TCR homodimer.

MeSH Terms
Animals Antigens, Differentiation, T-Lymphocyte/genetics Blotting, Northern CD3 Complex Calcium/metabolism Cell Membrane/metabolism Cells, Cultured Cross-Linking Reagents Flow Cytometry Mice Mice, SCID Mice, Transgenic Precipitin Tests Receptors, Antigen, T-Cell/genetics Receptors, Antigen, T-Cell, alpha-beta/biosynthesis,genetics Receptors, Antigen, T-Cell, gamma-delta/biosynthesis,genetics T-Lymphocytes/metabolism Transfection Tumor Cells, Cultured
Chemicals
Antigens, Differentiation, T-Lymphocyte CD3 Complex Cross-Linking Reagents Receptors, Antigen, T-Cell Receptors, Antigen, T-Cell, alpha-beta Receptors, Antigen, T-Cell, gamma-delta Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Groettrup M
Basel Institute for Immunology, Switzerland.
Baron A
Griffiths G
Palacios R
von Boehmer H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-07-00
Pages
2735-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556749
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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