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PMID: 14701739 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Coordination of DNA damage responses via the Smc5/Smc6 complex.

Molecular and cellular biology ·Vol. 24 ·No. 2 ·2004-01-00 ·Pages 662-74

Harvey SH, Sheedy DM, Cuddihy AR, O'Connell MJ

Abstract

The detection of DNA damage activates DNA repair pathways and checkpoints to allow time for repair. Ultimately, these responses must be coordinated to ensure that cell cycle progression is halted until repair is completed. Several multiprotein complexes containing members of the structural maintenance of chromosomes family of proteins have been described, including the condensin and cohesin complexes, that are critical for chromosomal organization. Here we show that the Smc5/Smc6 (Smc5/6) complex is required for a coordinated response to DNA damage and normal chromosome integrity. Fission yeast cells lacking functional Smc6 initiate a normal checkpoint response to DNA damage, culminating in the phosphorylation and activation of the Chk1 protein kinase. Despite this, cells enter a lethal mitosis, presumably without completion of DNA repair. Another subunit of the complex, Nse1, is a conserved member of this complex and is also required for this response. We propose that the failure to maintain a checkpoint response stems from the lack of ongoing DNA repair or from defective chromosomal organization, which is the signal to maintain a checkpoint arrest. The Smc5/6 complex is fundamental to genome integrity and may function with the condensin and cohesin complexes in a coordinated manner.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Cycle Cell Cycle Proteins/genetics,metabolism DNA Damage/genetics,physiology DNA Repair/genetics,physiology DNA, Fungal/genetics,metabolism Molecular Sequence Data Mutation Schizosaccharomyces/cytology,genetics,metabolism Schizosaccharomyces pombe Proteins/genetics,metabolism Sequence Homology, Amino Acid
Chemicals
Cell Cycle Proteins DNA, Fungal Schizosaccharomyces pombe Proteins Smc5 protein, S pombe smc6 protein, S pombe
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Harvey Susan H
Trescowthick Research Laboratories, Peter MacCallum Cancer Centre, Melbourne, Victoria 8006, Australia.
Sheedy Daniel M
Cuddihy Andrew R
O'Connell Matthew J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
Published
2004-01-00
Pages
662-74
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC343814
Subset
IM
Grants
NCI NIH HHS · R01 CA100076 · United States
NCI NIH HHS · R56 CA100076 · United States
NCI NIH HHS · CA 100076-01 · United States
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