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PMID: 15243061 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Prevalence of the hifBC, hmw1A, hmw2A, hmwC, and hia Genes in Haemophilus influenzae Isolates.

Journal of clinical microbiology ·Vol. 42 ·No. 7 ·2004-07-00 ·Pages 3065-72

Ecevit IZ, McCrea KW, Pettigrew MM, Sen A, Marrs CF, Gilsdorf JR

Abstract

Adherence of Haemophilus influenzae to respiratory epithelial cells is the first step in the pathogenesis of H. influenzae infection and is facilitated by the action of several adhesins located on the surface of the bacteria. In this study, prevalences of hifBC, which represent the pilus gene cluster; hmw1A, hmw2A, and hmwC, which represent high-molecular-weight (HMW) adhesin genes; and hia, which represents H. influenzae adhesin (Hia) genes were determined among clinical isolates of encapsulated type b (Hib) and nonencapsulated (NTHi) H. influenzae. hifBC genes were detected in 109 of 170 (64%) Hib strains and in 46 of 162 (28%) NTHi isolates (P = 0.0001) and were more prevalent among the invasive type b strains than invasive NTHi strains (P = 0.00003). Furthermore, hifBC genes were significantly more prevalent (P = 0.0398) among NTHi throat isolates than NTHi middle ear isolates. hmw1A, hmw2A, hmwC, and hia genes were not detected in Hib strains. Among NTHi isolates, the prevalence of hmw1A was 51%, the prevalence of hmw2A was 23%, the prevalence of hmwC was 48%, and the prevalence of hia was 33%. The hmw genes were significantly more prevalent among middle ear than throat isolates, while hia did not segregate with a respiratory tract site. These results show the variability of the presence of adhesin genes among clinical H. influenzae isolates and suggest that hemagglutinating pili may play a larger role in H. influenzae nasopharyngeal colonization than in acute otitis media whereas the HMW adhesins may be virulence factors for acute otitis media.

MeSH Terms
Adhesins, Bacterial/genetics Bacterial Outer Membrane Proteins/genetics Bacterial Proteins/genetics Genes, Bacterial Haemophilus influenzae/genetics Humans Molecular Chaperones/genetics Multigene Family Nasopharynx/microbiology
Chemicals
Adhesins, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins HMW1 protein, bacteria HMW2 protein, bacteria Hia adhesin HifB protein, Haemophilus influenzae HifC protein, Haemophilus influenzae Molecular Chaperones
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ecevit I Zafer
Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109-0244, USA.
McCrea Kirk W
Pettigrew Melinda M
Sen Ananda
Marrs Carl F
Gilsdorf Janet R
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.42.7.3065-3072.2004
Published
2004-07-00
Pages
3065-72
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC446296
Subset
IM
Grants
NIDCD NIH HHS · R01 DC005840 · United States
NIDCD NIH HHS · DC05840 · United States
External Links
PubMed source DOI: 10.1128/JCM.42.7.3065-3072.2004 PMC Full Text View journal details
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