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PMID: 1542567 Published · ppublish English Journal Article

Cloning and expression of the HpaI restriction-modification genes.

Nucleic acids research ·Vol. 20 ·No. 4 ·1992-02-25 ·Pages 705-9

Ito H, Shimato H, Sadaoka A, Kotani H, Kimizuka F, Kato I

Abstract

The genes from Haemophilus parainfluenzae encoding the HpaI restriction-modification system were cloned and expressed in Escherichia coli. From the DNA sequence, we predicted the HpaI endonuclease (R.HpaI) to have 254 amino acid residues (Mr 29,630) and the HpaI methyltransferase (M.HpaI) to have 314 amino acid residues (37,390). The R.HpaI and M.HpaI genes overlapped by 16 base pairs on the chromosomal DNA. The genes had the same orientation. The clone, named E. coli HB101-HPA2, overproduced R.HpaI. R.HpaI activity from the clone was 100-fold that from H. parainfluenzae. The amino acid sequence of M.HpaI was compared with those of other type II methyltransferases.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Southern Cloning, Molecular Deoxyribonucleases, Type II Site-Specific/chemistry,genetics Gene Expression Haemophilus/enzymology,genetics Molecular Sequence Data Restriction Mapping Site-Specific DNA-Methyltransferase (Adenine-Specific)/chemistry,genetics
Chemicals
DNA modification methylase HpaI Site-Specific DNA-Methyltransferase (Adenine-Specific) endodeoxyribonuclease HpaI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ito H
Bioproducts Development Center, Takara Shuzo Co., Ltd, Shiga, Japan.
Shimato H
Sadaoka A
Kotani H
Kimizuka F
Kato I
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.4.705
Published
1992-02-25
Pages
705-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312008
Subset
IM
Databases
GENBANK
D01166
External Links
PubMed source DOI: 10.1093/nar/20.4.705 PMC Full Text View journal details
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