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PMID: 3001639 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Nucleotide sequence of the PaeR7 restriction/modification system and partial characterization of its protein products.

Nucleic acids research ·Vol. 13 ·No. 23 ·1985-12-09 ·Pages 8441-61

Theriault G, Roy PH, Howard KA, Benner JS, Brooks JE, Waters AF, Gingeras TR

Abstract

Bal31 deletion experiments on clones of the PaeR7 restriction-modification system from Pseudomonas aeruginosa demonstrate that it is arranged as an operon, with the methylase gene preceding the endonuclease gene. The DNA sequence of this operon agrees with in vitro transcription-translation assays which predict proteins of 532 amino acids, Mr = 59,260 daltons, and 246 amino acids, Mr = 27,280 daltons, coincident with the methylase and endonuclease genes, respectively. These predicted values coincide with the measured molecular weights of the purified, denatured PaeR7 endonuclease and methylase proteins. The first twenty amino acids from the amino-terminus of the purified endonuclease exactly match those predicted from the DNA sequence. Finally, potential regulatory mechanisms for the expression of phage restriction are described based on the properties of several PaeR7 subclones.

MeSH Terms
Amino Acid Sequence Base Sequence Cell-Free System Cloning, Molecular DNA Restriction Enzymes/genetics,isolation & purification DNA-Cytosine Methylases Deoxyribonucleases, Type II Site-Specific Escherichia coli Genes Genes, Bacterial Methyltransferases/genetics,isolation & purification Plasmids Protein Biosynthesis Pseudomonas aeruginosa/genetics Transcription, Genetic
Chemicals
DNA modification methylase PaeR7 DNA-Cytosine Methylases Methyltransferases DNA Restriction Enzymes CTCGAG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Theriault G
Roy P H
Howard K A
Benner J S
Brooks J E
Waters A F
Gingeras T R
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38 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.23.8441
Published
1985-12-09
Pages
8441-61
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322144
Subset
IM
Databases
GENBANK
X03274
External Links
PubMed source DOI: 10.1093/nar/13.23.8441 PMC Full Text View journal details
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