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PMID: 2183182 Published · ppublish English Journal Article

Cloning and nucleotide sequence of the gene encoding the Ecal DNA methyltransferase.

Nucleic acids research ·Vol. 18 ·No. 2 ·1990-01-25 ·Pages 355-9

Brenner V, Venetianer P, Kiss A

Abstract

The gene coding for the GGTNACC specific Ecal DNA methyltransferase (M.Ecal) has been cloned in E. coli from Enterobacter cloacae and its nucleotide sequence has been determined. The ecalM gene codes for a protein of 452 amino acids (Mr: 51,111). It was determined that M.Ecal is an adenine methyltransferase. M.Ecal shows limited amino acid sequence similarity to other adenine methyltransferases. A clone that expresses Ecal methyltransferase at high level was constructed.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular/methods Electrophoresis, Polyacrylamide Gel Enterobacter/enzymology,genetics Enterobacteriaceae/genetics Escherichia coli/enzymology,genetics Genes, Bacterial Molecular Sequence Data Molecular Weight Plasmids Recombinant Proteins/isolation & purification,metabolism Restriction Mapping Site-Specific DNA-Methyltransferase (Adenine-Specific)/genetics,isolation & purification,metabolism Substrate Specificity
Chemicals
Recombinant Proteins DNA modification methylase EcaI Site-Specific DNA-Methyltransferase (Adenine-Specific)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brenner V
Institute of Biochemistry, Biological Research Center, Hungarian Academy of Sciences, Szeged.
Venetianer P
Kiss A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.2.355
Published
1990-01-25
Pages
355-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330275
Subset
IM
Databases
GENBANK
X17111
External Links
PubMed source DOI: 10.1093/nar/18.2.355 PMC Full Text View journal details
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