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PMID: 1556069 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dissection of IncP conjugative plasmid transfer: definition of the transfer region Tra2 by mobilization of the Tra1 region in trans.

Journal of bacteriology ·Vol. 174 ·No. 8 ·1992-04-00 ·Pages 2493-500

Lessl M, Balzer D, Lurz R, Waters VL, Guiney DG, Lanka E

Abstract

We constructed a transfer system consisting of two compatible multicopy plasmids carrying the transfer regions Tra1 and Tra2 of the broad-host-range IncP plasmid RP4. In this system, the plasmid containing the Tra1 region with the origin of transfer (oriT) was transferred, whereas additional functions essential for the conjugative process were provided from the Tra2 plasmid in trans. The Tra2 region, as determined for matings between Escherichia coli cells, maps between coordinates 18.03 and 29.26 kb of the RP4 standard map. The section of Tra2 required for mobilization of the plasmid RSF1010 (IncQ) and the propagation of bacteriophages Pf3 and PRD1 appears to be the same as that needed for RP4 transfer. Tra2 regions of RP4 (IncP alpha) and R751 (IncP beta) are interchangeable, facilitating mobilization of the plasmid carrying the RP4 Tra1 region. The transfer frequencies of both systems are similar. Transcription of Tra2 proceeds clockwise relative to the standard map of RP4 and is probably initiated at a promoter region located upstream of trbB (kilB). From this promoter region the trfA operon and the Tra2 operon are likely to be transcribed divergently. A second potential promoter has been located immediately upstream of trbB (kilB). Plasmids encoding the functional Tra2 region can only be maintained stably in host cells in the presence of the RP4 regulation region carrying the korA-korB operon or part of it. This indicates the involvement of RP4 key regulatory functions that apparently are active not only in the control of replication but also in conjugation.

Related Genes
aphA kfrA kilB korA korB korF tra1 tra2 trbA trbB
MeSH Terms
Base Sequence Cloning, Molecular Conjugation, Genetic DNA, Bacterial/genetics Escherichia coli/genetics Genes, Bacterial Molecular Sequence Data Plasmids Regulatory Sequences, Nucleic Acid Restriction Mapping
Chemicals
DNA, Bacterial
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lessl M
Max-Planck-Institut für Molekulare Genetik, Abteilung Schuster, Berlin, Germany.
Balzer D
Lurz R
Waters V L
Guiney D G
Lanka E
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51 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.8.2493-2500.1992
Published
1992-04-00
Pages
2493-500
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205887
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.8.2493-2500.1992 PMC Full Text View journal details
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