Home LiteratureArticle Details
PMID: 1904533 Published · ppublish English Journal Article

Identification and characterization of two entry exclusion genes of the promiscuous IncP plasmid R18.

Molecular & general genetics : MGG ·Vol. 227 ·No. 1 ·1991-05-00 ·Pages 120-6

Lessl M, Krishnapillai V, Schilf W

Abstract

Two entry exclusion genes (designated eexA and eexB) from the promiscuous IncP alpha plasmid R18 have been isolated by molecular cloning. They are located between coordinates 26.6-27.4 kb and 27.4-27.6 kb, respectively and are transcribed clockwise on the conventional R18 map. The product of the eexA gene has an apparent molecular mass of 28 kDa and its N-terminus contains a putative signal sequence for protein export. A recombinant plasmid containing R18 eex genes exerted Eex activity towards another promiscuous IncP alpha plasmid, R702, about 50 times more strongly than plasmid R18 itself. Analysis of the DNA sequence revealed no similarity to the eex genes of the F plasmid of Escherichia coli. R18 eexA includes a potential korB binding site and is followed by a potential transcription terminator. A Tn7 insertion at coordinate 20.0 kb of R18 resulted in a host range mutant pM01185, which leads to loss of Eex activity and of conjugative transfer of the plasmid into some bacterial species.

Related Genes
eex
MeSH Terms
Amino Acid Sequence Base Sequence DNA, Bacterial/genetics,isolation & purification Escherichia coli/genetics Genes, Bacterial Genotype Klebsiella pneumoniae/genetics Molecular Sequence Data Open Reading Frames Phenotype Pseudomonas/genetics Pseudomonas aeruginosa/genetics R Factors Restriction Mapping Transformation, Bacterial
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lessl M
Lehrstuhl für Genetik, Universität Bayreuth, FRG.
Krishnapillai V
Schilf W
References (44)
44 references, click to expand
  1. Plasmid-determined resistance to serum bactericidal activity: a major outer membrane protein, the traT gene product, is responsible for plasmid-specified serum resistance in Escherichia coli.
    Infect Immun. 1980 May;28(2):359-67 PMID: 6995306
  2. Adenine + thymine content of different genes located on the broad host range plasmid RP4.
    J Gen Microbiol. 1980 Mar;117(1):135-40 PMID: 6248619
  3. Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication.
    Gene. 1982 Jan;17(1):79-89 PMID: 6281139
  4. Identification of a membrane protein associated with expression of the surface exclusion region of the F transfer operon.
    J Bacteriol. 1977 Mar;129(3):1613-22 PMID: 321436
  5. Transcriptional analysis of the F plasmid surface exclusion region: mapping of traS, traT, and traD transcripts.
    Plasmid. 1989 Jan;21(1):1-8 PMID: 2657819
  6. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector.
    Gene. 1986;48(1):119-31 PMID: 3549457
  7. Uniform nomenclature for bacterial plasmids: a proposal.
    Bacteriol Rev. 1976 Mar;40(1):168-89 PMID: 1267736
  8. A computer algorithm for testing potential prokaryotic terminators.
    Nucleic Acids Res. 1984 May 25;12(10):4411-27 PMID: 6374619
  9. Control of Tn7 transposition.
    Mol Gen Genet. 1984;194(1-2):149-58 PMID: 6328211
  10. A genetic analysis of F sex factor cistrons needed for surface exclusion in Escherichia coli.
    J Mol Biol. 1980 Apr 25;138(4):779-95 PMID: 6997497
  11. Incompatibility group P plasmids: genetics, evolution, and use in genetic manipulation.
    Annu Rev Microbiol. 1987;41:77-101 PMID: 3318684
  12. Compilation of published signal sequences.
    Nucleic Acids Res. 1984 Jul 11;12(13):5145-64 PMID: 6379599
  13. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  14. Linkage map of Escherichia coli K-12, edition 8.
    Microbiol Rev. 1990 Jun;54(2):130-97 PMID: 2194094
  15. Complementation analysis in Pseudomonas aeruginosa of the transfer genes of the wide host range R plasmid R18.
    Plasmid. 1981 Mar;5(2):202-12 PMID: 6264523
  16. Analysis of E. coli promoter sequences.
    Nucleic Acids Res. 1987 Mar 11;15(5):2343-61 PMID: 3550697
  17. Effect of some R factors on the sensitivity of rough Enterobacteriaceae to human serum.
    Infect Immun. 1977 Nov;18(2):278-82 PMID: 336539
  18. A plasmid-encoded outer membrane protein, TraT, enhances resistance of Escherichia coli to phagocytosis.
    Infect Immun. 1984 Dec;46(3):740-6 PMID: 6389361
  19. Purification and characterization of pro-TraTp, the signal sequence-containing precursor of a secreted protein encoded by the F sex factor.
    J Bacteriol. 1984 May;158(2):464-73 PMID: 6373720
  20. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  21. The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences.
    Gene. 1984 Oct;30(1-3):157-66 PMID: 6096212
  22. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  23. Cell--cell interactions in conjugating Escherichia coli: role of traT protein in surface exclusion.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):5104-8 PMID: 337311
  24. Evidence that TraT interacts with OmpA of Escherichia coli.
    FEBS Lett. 1986 Sep 15;205(2):241-5 PMID: 3527751
  25. Nucleotide sequence of the surface exclusion genes traS and traT from the IncF0 lac plasmid pED208.
    J Bacteriol. 1986 Jun;166(3):713-21 PMID: 3011738
  26. The kil-kor regulon of broad-host-range plasmid RK2: nucleotide sequence, polypeptide product, and expression of regulatory gene korC.
    J Bacteriol. 1990 Jun;172(6):3040-50 PMID: 2160936
  27. Cloning and genetic analysis of tra cistrons of the Tra 2/Tra 3 region of plasmid RP1.
    Plasmid. 1989 Jul;22(1):59-69 PMID: 2550984
  28. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  29. Overproduction, purification and characterization of the F traT protein.
    Mol Gen Genet. 1984;196(2):225-35 PMID: 6238226
  30. Surface exclusion genes traS and traT of the F sex factor of Escherichia coli K-12. Determination of the nucleotide sequence and promoter and terminator activities.
    J Mol Biol. 1987 Nov 5;198(1):1-11 PMID: 3323526
  31. Relationship of group P1 plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical.
    J Gen Microbiol. 1979 Oct;114(2):341-8 PMID: 120408
  32. The product of the F sex factor traT surface exclusion gene is a lipoprotein.
    J Biol Chem. 1984 May 10;259(9):5357-60 PMID: 6325433
  33. Nucleotide sequence and organization of genes flanking the transfer origin of promiscuous plasmid RP4.
    DNA Seq. 1991;1(5):303-27 PMID: 1665997
  34. Improved free-energy parameters for predictions of RNA duplex stability.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9373-7 PMID: 2432595
  35. Pedigrees of some mutant strains of Escherichia coli K-12.
    Bacteriol Rev. 1972 Dec;36(4):525-57 PMID: 4568763
  36. A non-transmissible variant of RP4 suitable as cloning vehicle for genetic engineering.
    FEBS Lett. 1976 Jan 15;61(2):186-8 PMID: 765151
  37. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  38. The codon preference plot: graphic analysis of protein coding sequences and prediction of gene expression.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):539-49 PMID: 6694906
  39. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  40. Tn7 insertion mutations affecting the host range of the promiscuous IncP-1 plasmid R18.
    Plasmid. 1982 Sep;8(2):164-74 PMID: 6294704
  41. Molecular cloning and mapping of SphI restriction fragments of plasmid RP4.
    Plasmid. 1983 Nov;10(3):303-7 PMID: 6318250
  42. Insertion mutations in the promiscuous IncP-1 plasmid R18 which affect its host range between Pseudomonas species.
    Plasmid. 1984 Nov;12(3):170-80 PMID: 6098904
  43. Nucleotide sequence analysis of the complement resistance gene from plasmid R100.
    J Bacteriol. 1982 Aug;151(2):819-27 PMID: 6284713
  44. Comparison of 10 IncP plasmids: homology in the regions involved in plasmid replication.
    J Bacteriol. 1985 May;162(2):656-60 PMID: 2985542
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00260716
Published
1991-05-00
Pages
120-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X54103, X56839, X63980, X63981, X63982, X63983, X63984, X63985, X63986, X63987
External Links
PubMed source DOI: 10.1007/BF00260716
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2