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PMID: 15706350 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The yeast EDC1 mRNA undergoes deadenylation-independent decapping stimulated by Not2p, Not4p, and Not5p.

The EMBO journal ·Vol. 24 ·No. 5 ·2005-03-09 ·Pages 1033-45

Muhlrad D, Parker R

Abstract

A major mechanism of eukaryotic mRNA degradation initiates with deadenylation followed by decapping and 5' to 3' degradation. We demonstrate that the yeast EDC1 mRNA, which encodes a protein that enhances decapping, has unique properties and is both protected from deadenylation and undergoes deadenylation-independent decapping. The 3' UTR of the EDC1 mRNA is sufficient for both protection from deadenylation and deadenylation-independent decapping and an extended poly(U) tract within the 3' UTR is required. These observations highlight the diverse forms of decapping regulation and identify a feedback loop that can compensate for decreases in activity of the decapping enzyme. Surprisingly, the decapping of the EDC1 mRNA is slowed by the loss of Not2p, Not4p, and Not5p, which interact with the Ccr4p/Pop2p deadenylase complex. This indicates that the Not proteins can affect decapping, which suggests a possible link between the mRNA deadenylation and decapping machinery.

MeSH Terms
3' Untranslated Regions Base Sequence Cell Cycle Proteins/metabolism DNA, Fungal/genetics Molecular Sequence Data RNA Caps/chemistry,genetics,metabolism RNA, Fungal/chemistry,genetics,metabolism RNA, Messenger/chemistry,genetics,metabolism RNA-Binding Proteins Repressor Proteins/metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Transcription Factors/metabolism
Chemicals
3' Untranslated Regions CDC36 protein, S cerevisiae Cell Cycle Proteins DNA, Fungal Edc1 protein, S cerevisiae NOT5 protein, S cerevisiae RNA Caps RNA, Fungal RNA, Messenger RNA-Binding Proteins Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Muhlrad Denise
Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of Arizona, Tucson, AZ 85721, USA.
Parker Roy
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600560
Published
2005-03-09
Epub
2005-00-10
Pages
1033-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554118
Subset
IM
Grants
NIGMS NIH HHS · R37 GM045443 · United States
NIGMS NIH HHS · R37 GM045443-15 · United States
External Links
PubMed source DOI: 10.1038/sj.emboj.7600560 PMC Full Text View journal details
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