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PMID: 1587585 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of an RTX toxin determinant from Actinobacillus suis.

Infection and immunity ·Vol. 60 ·No. 6 ·1992-06-00 ·Pages 2166-73

Burrows LL, Lo RY

Abstract

RTX cytolysins are a family of calcium-dependent, pore-forming, secreted toxins found in a variety of gram-negative bacteria. The prototypical member of this family is the alpha-hemolysin of Escherichia coli. The RTX genetic determinants from seven members of the family Pasteurellaceae, Pasteurella haemolytica, Actinobacillus actinomycetemcomitans, and A. pleuropneumoniae serotypes 1,5,7, and 9 were previously cloned and sequenced. Using the leukotoxin determinant from P. haemolytica serotype A1 as a probe, we detected the presence of RTX-type determinants in Actinobacillus suis, A. equuli, and A. lignieresii of the family Pasteurellaceae. All three species elaborate proteins of approximately 104 to 110 kDa that are recognized by polyclonal antisera against the 104-kDa hemolysin of A. pleuropneumoniae serotype 1. An RTX determinant of A. suis isolate 3714 was cloned and sequenced and was found to be almost identical to the RTX determinant of A. pleuropneumoniae serotypes 5 and 9. In addition, the determinant is not composed of four contiguous genes, as had been reported for most other RTX determinants; instead, the genes encoding the two proteins responsible for secretion of the toxin are at a locus distinct from that containing the toxin structural and activation genes.

MeSH Terms
Actinobacillus/genetics,metabolism Amino Acid Sequence Animals Bacterial Toxins/genetics Base Sequence Blotting, Southern Blotting, Western Cloning, Molecular Cytotoxins/genetics Hemolysin Proteins/genetics Hemolysis Humans Molecular Sequence Data
Chemicals
Bacterial Toxins Cytotoxins Hemolysin Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Burrows L L
Department of Microbiology, University of Guelph, Ontario, Canada.
Lo R Y
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33 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.60.6.2166-2173.1992
Published
1992-06-00
Pages
2166-73
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC257139
Subset
IM
Databases
GENBANK
M63610, M63611, M63612, M63613, M63614, M63615, M63616, M63617, M90440, X63185
External Links
PubMed source DOI: 10.1128/iai.60.6.2166-2173.1992 PMC Full Text View journal details
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