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PMID: 1592821 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Mutational analysis reveals functional similarity between NARX, a nitrate sensor in Escherichia coli K-12, and the methyl-accepting chemotaxis proteins.

Journal of bacteriology ·Vol. 174 ·No. 11 ·1992-06-00 ·Pages 3667-75

Collins LA, Egan SM, Stewart V

Abstract

During anaerobic growth, nitrate induces synthesis of the anaerobic respiratory enzymes formate dehydrogenase-N and nitrate reductase. This induction is mediated by a transcription activator, the narL gene product. The narX gene product may be involved in sensing nitrate and phosphorylating NARL. We isolated narX mutants, designated narX*, that caused nitrate-independent expression of the formate dehydrogenase-N and nitrate reductase structural genes. We used lambda narX specialized transducing phage to genetically analyze these lesions in single copy. Two previously isolated narX* mutations, narX32 and narX71, were also constructed by site-specific mutagenesis. We found that each of these alleles caused nitrate-independent synthesis of formate dehydrogenase-N and nitrate reductase, and each was recessive to narX+. The narX* mutations lie in a region of similarity with the methyl-accepting chemotaxis protein Tsr. We suggest that the narX* proteins have lost a transmembrane signalling function such that phosphoprotein phosphatase activity is reduced relative to protein kinase activity.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence DNA Mutational Analysis Escherichia coli/genetics Escherichia coli Proteins Gene Expression Regulation, Bacterial/drug effects,genetics Genetic Complementation Test Membrane Proteins/genetics Methyl-Accepting Chemotaxis Proteins Molecular Sequence Data Molybdenum/pharmacology Mutagenesis Mutation/genetics Nitrates/pharmacology Phenotype Protein Kinases Sequence Alignment/methods Sequence Homology, Nucleic Acid Signal Transduction/drug effects,genetics
Chemicals
Bacterial Proteins Escherichia coli Proteins Membrane Proteins Methyl-Accepting Chemotaxis Proteins Nitrates Tsr protein, Bacteria tsr protein, E coli molybdate Molybdenum Protein Kinases narX protein, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Collins L A
Section of Genetics, Cornell University, Ithaca, New York 14853-8101.
Egan S M
Stewart V
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.11.3667-3675.1992
Published
1992-06-00
Pages
3667-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206056
Subset
IM
Grants
NIGMS NIH HHS · R01 GM36877 · United States
NIGMS NIH HHS · T32 GM07617 · United States
External Links
PubMed source DOI: 10.1128/jb.174.11.3667-3675.1992 PMC Full Text View journal details
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