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PMID: 1594612 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning of a Rab3 isotype predominantly expressed in adipocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 11 ·1992-06-01 ·Pages 5049-52

Baldini G, Hohl T, Lin HY, Lodish HF

Abstract

We have isolated the cDNA for Rab3D, an additional member of the small molecular weight GTP-binding protein family. Rab3D message is abundant in mouse adipocytes. It is increased during differentiation of 3T3-L1 cells into adipocytes, temporally coincident with the appearance of the insulin-sensitive glucose transporter GLUT4. Rab3D is a close homolog of Rab3A, which is found on the cytoplasmic surface of neurosecretory vesicles and which may be involved in their regulated secretion. Since our previous work showed that in permeabilized adipocytes nonhydrolizable GTP analogs mimic insulin in triggering exocytosis of GLUT4-containing vesicles, Rab3D may be involved in the insulin-induced exocytosis of GLUT4-containing vesicles in adipocytes.

MeSH Terms
3T3 Cells Adipose Tissue/physiology Amino Acid Sequence Animals Base Sequence Cell Differentiation Cloning, Molecular GTP-Binding Proteins/genetics Gene Expression Mice Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Polymerase Chain Reaction RNA, Messenger/genetics Sequence Alignment Species Specificity Transcription, Genetic rab3 GTP-Binding Proteins
Chemicals
Oligodeoxyribonucleotides RNA, Messenger GTP-Binding Proteins rab3 GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baldini G
Whitehead Institute for Biomedical Research, Nine Cambridge Center, MA 02142.
Hohl T
Lin H Y
Lodish H F
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.11.5049
Published
1992-06-01
Pages
5049-52
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49226
Subset
IM
Grants
NIGMS NIH HHS · GM-49516 · United States
NHLBI NIH HHS · HL-41484 · United States
Databases
GENBANK
M89777
External Links
PubMed source DOI: 10.1073/pnas.89.11.5049 PMC Full Text View journal details
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