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PMID: 15964821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein.

Molecular and cellular biology ·Vol. 25 ·No. 13 ·2005-07-00 ·Pages 5664-74

Nishi R, Okuda Y, Watanabe E, Mori T, Iwai S, Masutani C, Sugasawa K, Hanaoka F

Abstract

Xeroderma pigmentosum group C (XPC) protein plays a key role in DNA damage recognition in global genome nucleotide excision repair (NER). The protein forms in vivo a heterotrimeric complex involving one of the two human homologs of Saccharomyces cerevisiae Rad23p and centrin 2, a centrosomal protein. Because centrin 2 is dispensable for the cell-free NER reaction, its role in NER has been unclear. Binding experiments with a series of truncated XPC proteins allowed the centrin 2 binding domain to be mapped to a presumed alpha-helical region near the C terminus, and three amino acid substitutions in this domain abrogated interaction with centrin 2. Human cell lines stably expressing the mutant XPC protein exhibited a significant reduction in global genome NER activity. Furthermore, centrin 2 enhanced the cell-free NER dual incision and damaged DNA binding activities of XPC, which likely require physical interaction between XPC and centrin 2. These results reveal a novel vital function for centrin 2 in NER, the potentiation of damage recognition by XPC.

MeSH Terms
Amino Acid Motifs Amino Acid Sequence Calcium-Binding Proteins Cell Cycle Proteins/chemistry,metabolism Cell Line Conserved Sequence DNA/genetics,metabolism DNA Damage/radiation effects DNA Repair DNA-Binding Proteins/chemistry,genetics,metabolism Humans Molecular Sequence Data Mutation Protein Binding Protein Structure, Tertiary Sequence Homology, Amino Acid Ultraviolet Rays Xeroderma Pigmentosum/genetics,metabolism
Chemicals
CETN2 protein, human Calcium-Binding Proteins Cell Cycle Proteins DNA-Binding Proteins XPC protein, human DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Nishi Ryotaro
Cellular Physiology Laboratory, RIKEN Discovery Research Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Okuda Yuki
Watanabe Eriko
Mori Toshio
Iwai Shigenori
Masutani Chikahide
Sugasawa Kaoru
Hanaoka Fumio
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.25.13.5664-5674.2005
Published
2005-07-00
Pages
5664-74
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1156980
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.25.13.5664-5674.2005 PMC Full Text View journal details
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