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PMID: 16009724 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The WD40 protein Caf4p is a component of the mitochondrial fission machinery and recruits Dnm1p to mitochondria.

The Journal of cell biology ·Vol. 170 ·No. 2 ·2005-07-18 ·Pages 237-48

Griffin EE, Graumann J, Chan DC

Abstract

The mitochondrial division machinery regulates mitochondrial dynamics and consists of Fis1p, Mdv1p, and Dnm1p. Mitochondrial division relies on the recruitment of the dynamin-related protein Dnm1p to mitochondria. Dnm1p recruitment depends on the mitochondrial outer membrane protein Fis1p. Mdv1p interacts with Fis1p and Dnm1p, but is thought to act at a late step during fission because Mdv1p is dispensable for Dnm1p localization. We identify the WD40 repeat protein Caf4p as a Fis1p-associated protein that localizes to mitochondria in a Fis1p-dependent manner. Caf4p interacts with each component of the fission apparatus: with Fis1p and Mdv1p through its NH2-terminal half and with Dnm1p through its COOH-terminal WD40 domain. We demonstrate that mdv1delta yeast contain residual mitochondrial fission due to the redundant activity of Caf4p. Moreover, recruitment of Dnm1p to mitochondria is disrupted in mdv1delta caf4delta yeast, demonstrating that Mdv1p and Caf4p are molecular adaptors that recruit Dnm1p to mitochondrial fission sites. Our studies support a revised model for assembly of the mitochondrial fission apparatus.

MeSH Terms
Adaptor Proteins, Signal Transducing Carrier Proteins/genetics,metabolism GTP Phosphohydrolases/genetics,metabolism Mitochondria/genetics,physiology Mitochondrial Proteins/genetics,metabolism Mutation Protein Structure, Tertiary Protein Transport Saccharomyces cerevisiae/genetics,physiology,ultrastructure Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
Adaptor Proteins, Signal Transducing Carrier Proteins FIS1 protein, S cerevisiae MDV1 protein, S cerevisiae Mitochondrial Proteins Saccharomyces cerevisiae Proteins GTP Phosphohydrolases DNM1 protein, S cerevisiae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Griffin Erik E
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Graumann Johannes
Chan David C
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200503148
Published
2005-07-18
Epub
2005-00-11
Pages
237-48
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171414
Subset
IM
Grants
NIGMS NIH HHS · R01 GM062967 · United States
NIGMS NIH HHS · T32 GM007616 · United States
NIGMS NIH HHS · GM07616 · United States
NIGMS NIH HHS · GM62967 · United States
External Links
PubMed source DOI: 10.1083/jcb.200503148 PMC Full Text View journal details
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