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PMID: 1602563 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human chromosome 12 is required for optimal interactions between Tat and TAR of human immunodeficiency virus type 1 in rodent cells.

Journal of virology ·Vol. 66 ·No. 7 ·1992-07-00 ·Pages 4617-21

Alonso A, Derse D, Peterlin BM

Abstract

Levels of trans activation of the human immunodeficiency virus type 1 long terminal repeat (HIV-1 LTR) by the virally encoded transactivator Tat show marked species-specific differences. For example, levels of transactivation observed in Chinese hamster ovary (CHO) rodent cells are 10-fold lower than those in human cells or in CHO cells that contain the human chromosome 12. Thus, the human chromosome 12 codes for a protein or proteins that are required for optimal Tat activity. Here, the function of these cellular proteins was analyzed by using a number of modified HIV-1 LTRs and Tats. Neither DNA-binding proteins that bind to the HIV-1 LTR nor proteins that interact with the activation domain of Tat could be implicated in this defect. However, since species-specific differences were no longer observed with hybrid proteins that contain the activation domain of Tat fused to heterologous RNA-binding proteins, optimal interactions between Tat and the trans-acting responsive RNA (TAR) must depend on this factor(s).

MeSH Terms
Animals Base Sequence CHO Cells Chromosomes, Human, Pair 12 Cricetinae DNA, Viral Gene Products, tat/metabolism HIV Long Terminal Repeat HIV-1/genetics,metabolism Humans Molecular Sequence Data Transcriptional Activation tat Gene Products, Human Immunodeficiency Virus
Chemicals
DNA, Viral Gene Products, tat tat Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Alonso A
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
Derse D
Peterlin B M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.7.4617-4621.1992
Published
1992-07-00
Pages
4617-21
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241279
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.7.4617-4621.1992 PMC Full Text View journal details
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