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PMID: 1645777 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of lentivirus tat functional domains through generation of equine infectious anemia virus/human immunodeficiency virus type 1 tat gene chimeras.

Journal of virology ·Vol. 65 ·No. 7 ·1991-07-00 ·Pages 3460-7

Carroll R, Martarano L, Derse D

Abstract

The structural regions that comprise the functional domains of lentivirus Tat proteins were examined. Chimeric tat genes and chimeric viral promoters were constructed between the distantly related human immunodeficiency virus type 1 (HIV-1) and equine infectious anemia virus (EIAV). These exchange experiments revealed that the EIAV Tat-responsive element recognition domain is formed by two distinct structural regions. Activation domains of both HIV-1 and EIAV Tat contain a conserved core element, but at least HIV-1 Tat requires the presence of additional structural regions. The interchangeable nature of Tat activation domains suggests that these domains act through a common or ubiquitous cellular transcription factor.

Related Genes
tat
MeSH Terms
Amino Acid Sequence Cloning, Molecular DNA Mutational Analysis Gene Expression Regulation, Viral Gene Products, tat/genetics HIV Long Terminal Repeat/genetics HIV-1/genetics Infectious Anemia Virus, Equine/genetics Lentivirus/genetics Molecular Sequence Data Promoter Regions, Genetic Recombinant Fusion Proteins Regulatory Sequences, Nucleic Acid Restriction Mapping Structure-Activity Relationship Transcriptional Activation tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat Recombinant Fusion Proteins tat Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Carroll R
Laboratory of Viral Carcinogenesis, NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201.
Martarano L
Derse D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.7.3460-3467.1991
Published
1991-07-00
Pages
3460-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241330
Subset
IM
Grants
NCI NIH HHS · N01-CO-74102 · United States
External Links
PubMed source DOI: 10.1128/JVI.65.7.3460-3467.1991 PMC Full Text View journal details
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