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PMID: 16140985 Published · ppublish English Journal Article

Genetic interactions of separase regulatory subunits reveal the diverged Drosophila Cenp-C homolog.

Genes & development ·Vol. 19 ·No. 17 ·2005-09-01 ·Pages 2041-53

Heeger S, Leismann O, Schittenhelm R, Schraidt O, Heidmann S, Lehner CF

Abstract

Faithful transmission of genetic information during mitotic divisions depends on bipolar attachment of sister kinetochores to the mitotic spindle and on complete resolution of sister-chromatid cohesion immediately before the metaphase-to-anaphase transition. Separase is thought to be responsible for sister-chromatid separation, but its regulation is not completely understood. Therefore, we have screened for genetic loci that modify the aberrant phenotypes caused by overexpression of the regulatory separase complex subunits Pimples/securin and Three rows in Drosophila. An interacting gene was found to encode a constitutive centromere protein. Characterization of its centromere localization domain revealed the presence of a diverged CENPC motif. While direct evidence for an involvement of this Drosophila Cenp-C homolog in separase activation at centromeres could not be obtained, in vivo imaging clearly demonstrated that it is required for normal attachment of kinetochores to the spindle.

MeSH Terms
Animals Animals, Genetically Modified Cell Cycle Proteins/chemistry,genetics Centromere/enzymology,genetics Chromosomal Proteins, Non-Histone/chemistry,genetics Drosophila/cytology,enzymology,genetics Drosophila Proteins/chemistry,genetics Endopeptidases/chemistry,genetics Eye Abnormalities/genetics Genes, Insect Kinetochores/enzymology Mitosis/genetics Mutation Phenotype Protein Structure, Tertiary Protein Subunits Separase Spindle Apparatus/enzymology,genetics Wings, Animal/abnormalities,growth & development
Chemicals
CENP-C protein, Drosophila Cell Cycle Proteins Chromosomal Proteins, Non-Histone Drosophila Proteins Protein Subunits centromere protein C Endopeptidases Separase Sse protein, Drosophila
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Heeger Sebastian
Department of Genetics, BZMB, University of Bayreuth, 95440 Bayreuth, Germany.
Leismann Oliver
Schittenhelm Ralf
Schraidt Oliver
Heidmann Stefan
Lehner Christian F
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.347805
Published
2005-09-01
Pages
2041-53
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1199574
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.347805 PMC Full Text View journal details
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