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PMID: 16151844 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression analyses of Arabidopsis oligopeptide transporters during seed germination, vegetative growth and reproduction.

Planta ·Vol. 223 ·No. 2 ·2006-01-00 ·Pages 291-305

Stacey MG, Osawa H, Patel A, Gassmann W, Stacey G

Abstract

AtOPT promoter-GUS fusions were constructed for six of the nine known, putative oligopeptide transporters (OPTs) in Arabidopsis thaliana and used to examine AtOPT expression at various stages of plant development. AtOPT1, AtOPT3, AtOPT4, AtOPT6 and AtOPT7 were expressed in the embryonic cotyledons prior to root radicle emergence. Except for AtOPT8, which gave weak expression, all AtOPTs were strongly expressed in post-germinative seedlings with strongest expression in vascular tissues of cotyledons and hypocotyls. Preferential expression of AtOPTs in vascular tissues was also observed in cotyledons, leaves, hypocotyls, roots, flowers, siliques, and seed funiculi of seedlings and adult plants. Differential tissue-specific expression was observed for specific AtOPTs. For example, AtOPT1, AtOPT3 and AtOPT8 were uniquely expressed in pollen. Only AtOPT1 was expressed in growing pollen tubes, while only AtOPT6 was observed in ovules. AtOPT8 was transiently expressed in seeds during early stages of embryogenesis. Iron limitation was found to enhance expression of AtOPT3. These data suggest distinct cellular roles for specific AtOPTs including nitrogen mobilization during germination and senescence, pollen tube growth, pollen and ovule development, seed formation and metal transport.

MeSH Terms
Arabidopsis/embryology,growth & development,physiology Arabidopsis Proteins/metabolism Biological Transport, Active/physiology Flowers/anatomy & histology,growth & development,metabolism Genes, Reporter Germination/physiology Iron/metabolism Membrane Transport Proteins/metabolism Oligopeptides/metabolism Plants, Genetically Modified/anatomy & histology,embryology,physiology Pollen/metabolism Promoter Regions, Genetic Recombinant Fusion Proteins/metabolism Reproduction Seedlings/anatomy & histology,growth & development,metabolism Seeds/growth & development,metabolism Sequence Analysis, DNA Symporters/metabolism
Chemicals
Arabidopsis Proteins Membrane Transport Proteins OPT1 protein, Arabidopsis OPT3 protein, Arabidopsis OPT6 protein, Arabidopsis OPT8 protein, Arabidopsis Oligopeptides Recombinant Fusion Proteins Symporters Iron
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Stacey Minviluz G
Division of Plant Sciences, The University of Missouri, Columbia, MO 65211, USA.
Osawa Hiroki
Patel Ami
Gassmann Walter
Stacey Gary
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-005-0087-x
Published
2006-01-00
Epub
2005-00-08
Pages
291-305
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-005-0087-x View journal details
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