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PMID: 16237007 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptome and physiological responses to hydrogen peroxide of the facultatively phototrophic bacterium Rhodobacter sphaeroides.

Journal of bacteriology ·Vol. 187 ·No. 21 ·2005-11-00 ·Pages 7232-42

Zeller T, Moskvin OV, Li K, Klug G, Gomelsky M

Abstract

The transcriptome responses to hydrogen peroxide, H2O2, of the facultatively phototrophic bacterium Rhodobacter sphaeroides grown under semiaerobic conditions were investigated. At 7 min after the addition of 1 mM H2O2, the expression of approximately 9% of all genes (total, 394) was changed reliably by at least twofold. At 30 min, the number of genes (total, 88) and the magnitude of expression changes were much lower, indicating rapid recovery from stress. Two types of responses were observed: (i) an H2O2 stress response per se and (ii) a shift to high-oxygen metabolism. The former response involved the upregulation of genes for H2O2 detoxification, protein folding and proteolysis, DNA damage repair, iron transport and storage, iron-sulfur cluster repair, and the downregulation of genes for protein translation, motility, and cell wall and lipopolysaccharide synthesis. The shift to high-oxygen metabolism was evident from the differential regulation of genes for aerobic electron transport chain components and the downregulation of tetrapyrrole biosynthesis and photosystem genes. The abundance of photosynthetic complexes was decreased upon prolonged exposure of R. sphaeroides to H2O2, thus confirming the physiological significance of the transcriptome data. The regulatory pathways mediating the shift to high-oxygen metabolism were investigated. They involved the anaerobic activator FnrL and the antirepressor-repressor AppA-PpsR system. The transcription of FnrL-dependent genes was down at 7 min, apparently due to the transient inactivation by H2O2 of the iron-sulfur cluster of FnrL. The transcription of the AppA-PpsR-dependent genes was down at 30 min, apparently due to the significant decrease in appA mRNA.

MeSH Terms
Adaptation, Physiological Anti-Bacterial Agents/metabolism,pharmacology Bacterial Proteins/biosynthesis,genetics,metabolism Chemotaxis/genetics DNA Repair Flagella/genetics,metabolism Flavoproteins/genetics Gene Expression Profiling Gene Expression Regulation, Bacterial Hydrogen Peroxide/metabolism,pharmacology Oligonucleotide Array Sequence Analysis Oxidative Stress Oxygen/metabolism Polysaccharides, Bacterial/biosynthesis RNA, Bacterial/analysis RNA, Messenger/analysis Rhodobacter sphaeroides/drug effects,genetics,metabolism,physiology Tetrapyrroles/biosynthesis Trans-Activators/metabolism Transcription, Genetic
Chemicals
Anti-Bacterial Agents AppA protein, Rhodobacter sphaeroides Bacterial Proteins Flavoproteins FnrL protein, Rhodobacter sphaeroides Polysaccharides, Bacterial RNA, Bacterial RNA, Messenger Tetrapyrroles Trans-Activators Hydrogen Peroxide Oxygen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zeller Tanja
Institut für Mikrobiologie und Molekularbiologie, University of Giessen, Germany.
Moskvin Oleg V
Li Kuanyu
Klug Gabriele
Gomelsky Mark
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.187.21.7232-7242.2005
Published
2005-11-00
Pages
7232-42
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1272974
Subset
IM
Grants
NCRR NIH HHS · P20 RR015640 · United States
NCRR NIH HHS · P20 RR15640 · United States
External Links
PubMed source DOI: 10.1128/JB.187.21.7232-7242.2005 PMC Full Text View journal details
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