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PMID: 1624444 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequences of genes encoding penicillin-binding proteins from Streptococcus pneumoniae and Streptococcus oralis with high homology to Escherichia coli penicillin-binding proteins 1a and 1b.

Journal of bacteriology ·Vol. 174 ·No. 13 ·1992-07-00 ·Pages 4517-23

Martin C, Briese T, Hakenbeck R

Abstract

The nucleotide sequence of a 3,378-bp DNA fragment of Streptococcus pneumoniae that included the structural gene for penicillin-binding protein (PBP) 1a (ponA), which encodes 719 amino acids, was determined. Homologous DNA fragments from an S. oralis strain were amplified with ponA-specific oligonucleotides. The 2,524-bp S. oralis sequence contained the coding region for the first 636 amino acids of a PBP. The coding sequence differed by 437 nucleotides (27%) and one additional triplet, resulting in 87 amino acid substitutions (14%), from S. pneumoniae PBP 1a. Both PBPs are highly homologous to bifunctional high-M(r) Escherichia coli PBPs 1a and 1b.

Related Genes
ponA
MeSH Terms
Amino Acid Sequence Bacterial Proteins Base Sequence Carrier Proteins/genetics Cloning, Molecular DNA, Bacterial/genetics,isolation & purification Escherichia coli/enzymology,genetics Genes, Bacterial Hexosyltransferases/genetics Molecular Sequence Data Multienzyme Complexes/genetics Muramoylpentapeptide Carboxypeptidase/genetics Oligodeoxyribonucleotides Open Reading Frames Penicillin-Binding Proteins Penicillins/metabolism Peptidyl Transferases/genetics Polymerase Chain Reaction/methods Restriction Mapping Sequence Homology, Nucleic Acid Streptococcus/genetics Streptococcus pneumoniae/genetics
Chemicals
Bacterial Proteins Carrier Proteins DNA, Bacterial Multienzyme Complexes Oligodeoxyribonucleotides Penicillin-Binding Proteins Penicillins Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Martin C
Max-Planck Institut für molekulare Genetik, Berlin, Germany.
Briese T
Hakenbeck R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.13.4517-4523.1992
Published
1992-07-00
Pages
4517-23
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206242
Subset
IM
Databases
GENBANK
M79367, M79368, M79369, M79370, M79371, M79372, M84980, M90527, M90528, Z11493
External Links
PubMed source DOI: 10.1128/jb.174.13.4517-4523.1992 PMC Full Text View journal details
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