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PMID: 1630459 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation and expression of a growth arrest-specific gene (gas5) during growth, differentiation, and development.

Molecular and cellular biology ·Vol. 12 ·No. 8 ·1992-08-00 ·Pages 3514-21

Coccia EM, Cicala C, Charlesworth A, Ciccarelli C, Rossi GB, Philipson L, Sorrentino V

Abstract

The growth arrest-specific gas5 gene was isolated from mouse genomic DNA and structurally characterized. The transcriptional unit is divided into 12 exons that span around 7 kb. An alternative splicing mechanism gives rise to two mature mRNAs which contain either 11 or 12 exons, and both are found in the cytoplasm of growth-arrested cells. In vivo, the gas5 gene is ubiquitously expressed in mouse tissues during development and adult life. In Friend leukemia and NIH 3T3 cells, the levels of gas5 gene mRNA were high in saturation density-arrested cells and almost undetectable in actively growing cells. Run-on experiments indicated that the gas5 gene is transcribed at the same level in both growing and arrested cells. On the other hand, in dimethyl sulfoxide-induced differentiating cells a sharp decrease in the rate of transcription was observed shortly before the cells reached the postmitotic stage. These results indicate that in density-arrested cells accumulation of gas5 mRNA is controlled at the posttranscriptional level while in differentiating cells expression is regulated transcriptionally.

Related Genes
gas5
MeSH Terms
3T3 Cells Amino Acid Sequence Animals Base Sequence Cell Differentiation Cell Line Cloning, Molecular DNA/genetics,isolation & purification Embryo, Mammalian/physiology Embryonic and Fetal Development Exons Fetus/physiology Gene Expression Gene Expression Regulation Genes Leukemia, Experimental Mice Molecular Sequence Data Protein Biosynthesis RNA, Messenger/genetics Transcription, Genetic Transfection
Chemicals
RNA, Messenger DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Coccia E M
European Molecular Biology Laboratory, Heidelberg, Germany.
Cicala C
Charlesworth A
Ciccarelli C
Rossi G B
Philipson L
Sorrentino V
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.8.3514-3521.1992
Published
1992-08-00
Pages
3514-21
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364604
Subset
IM
Databases
GENBANK
X59728, X59729
External Links
PubMed source DOI: 10.1128/mcb.12.8.3514-3521.1992 PMC Full Text View journal details
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