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PMID: 16307366 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamic histone acetylation of late embryonic genes during seed germination.

Plant molecular biology ·Vol. 59 ·No. 6 ·2005-12-00 ·Pages 909-25

Tai HH, Tai GC, Beardmore T

Abstract

Histone acetylation is involved in the regulation of gene expression in plants and eukaryotes. Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from histones, which is associated with the repression of gene expression. To study the role of histone acetylation in the regulation of gene expression during seed germination, trichostatin A (TSA), a specific inhibitor of histone deacetylase, was used to treat imbibing Arabidopsis thaliana seeds. GeneChip arrays were used to show that TSA induces up-regulation of 45 genes and down-regulation of 27 genes during seed germination. Eight TSA-up-regulated genes were selected for further analysis - RAB18, RD29B, ATEM1, HSP70 and four late embryogenesis abundant protein genes (LEA). A gene expression time course shows that these eight genes are expressed at high levels in the dry seed and repressed upon seed imbibition at an exponential rate. In the presence of TSA, the onset of repression of the eight genes is not affected but the final level of repressed expression is elevated. Chromatin immunoprecipitation and HDAC assays show that there is a transient histone deacetylation event during seed germination at 1 day after imbibition, which serves as a key developmental signal that affects the repression of the eight genes.

MeSH Terms
Acetylation Analysis of Variance Arabidopsis/genetics,metabolism Chromatin/metabolism Chromatin Immunoprecipitation DNA/metabolism Down-Regulation Gene Expression Regulation, Plant Germination Histone Acetyltransferases/metabolism Histone Deacetylases/metabolism Histones/metabolism Hydroxamic Acids/chemistry,metabolism Models, Genetic Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Promoter Regions, Genetic RNA/chemistry,metabolism RNA, Complementary/metabolism RNA, Messenger/metabolism Reverse Transcriptase Polymerase Chain Reaction Seeds/metabolism Time Factors Up-Regulation
Chemicals
Chromatin Histones Hydroxamic Acids RNA, Complementary RNA, Messenger trichostatin A RNA DNA Histone Acetyltransferases Histone Deacetylases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tai Helen H
Canadian Forest Service, Natural Resources Canada, P.O. Box 4000, E3B 5P7, Fredericton, NB, Canada. [email protected]
Tai George C C
Beardmore Tannis
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-005-2081-x
Email
[email protected]
Published
2005-12-00
Pages
909-25
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/s11103-005-2081-x View journal details
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