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PMID: 16453848 Published · ppublish English Journal Article

Novel mechanisms for maturation of chloroplast transfer RNA precursors.

The EMBO journal ·Vol. 7 ·No. 6 ·1988-06-00 ·Pages 1567-74

Wang MJ, Davis NW, Gegenheimer P

Abstract

Despite the prokaryotic origins of chloroplasts, a plant chloroplast tRNA precursor is processed in a homologous in vitro system by a pathway distinct from that observed in Escherichia coli, but identical to that utilized for maturation of nuclear pre-tRNAs. The mature tRNA 5' terminus is generated by the site-specific endonucleolytic cleavage of an RNase P (or P-type) activity. The 3' end is likewise produced by a single precise endonucleolytic cut at the 3' terminus of the encoded tRNA domain. This is the first complete structural characterization of an organellar tRNA processing system using a homologous substrate. In contrast to eubacterial RNase P, chloroplast RNase P does not appear to contain an RNA subunit. The chloroplast activity bands with bulk protein at 1.28 g/ml in CsCI density gradients, whereas E.coli RNase P bands as ribonucleoprotein at 1.73 g/ml. Chloroplast RNase P activity survives treatment with micrococcal nuclease (MN) at levels 10- to 100-fold higher than those required to totally inactivate the E.coli enzyme. The chloroplast system is sensitive to a suppression of tRNA processing, caused by binding of inactive MN to pre-tRNA substrate, which is readily overcome by addition of carrier RNA to the assay.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wang M J
Department of Biochemistry, University of Kansas, Lawrence, KS 66045, USA.
Davis N W
Gegenheimer P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-06-00
Pages
1567-74
Language
English
Region
England
NLM ID
8208664
PMCID
PMC457138
External Links
PubMed source PMC Full Text View journal details
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