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PMID: 1648556 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Conjugative transposition of Tn916 requires the excisive and integrative activities of the transposon-encoded integrase.

Journal of bacteriology ·Vol. 173 ·No. 14 ·1991-07-00 ·Pages 4347-52

Storrs MJ, Poyart-Salmeron C, Trieu-Cuot P, Courvalin P

Abstract

Transposon Tn916 is a 16.4-kb broad-host-range conjugative transposon originally detected in the chromosome of Enterococcus faecalis DS16. Transposition of Tn916 and related transposons involves excision of a free, nonreplicative, covalently closed circular intermediate that is substrate for integration. Excisive recombination requires two transposon-encoded proteins, Xis-Tn and Int-Tn, whereas the latter protein alone is sufficient for integration. Here we report that conjugative transposition of Tn916 requires the presence of a functional integrase in both donor and recipient strains. We have constructed a mutant, designated Tn916-int1, by replacing the gene directing synthesis of Int-Tn by an allele inactivated in vitro. In mating experiments, transfer of Tn916-int1 from Bacillus subtilis to E. faecalis was detected only when the transposon-encoded integrase was supplied by trans-complementation in both the donor and the recipient. These results suggest that conjugative transposition of Tn916 requires circularization of the element in the donor followed by transfer and integration of the nonreplicative intermediate in the recipient.

MeSH Terms
Bacillus subtilis/enzymology,genetics Base Sequence Chromosomes, Bacterial Conjugation, Genetic DNA Nucleotidyltransferases/genetics,metabolism DNA Transposable Elements DNA, Bacterial/genetics,isolation & purification Enterococcus faecalis/enzymology,genetics Escherichia coli/genetics Integrases Molecular Sequence Data Oligonucleotide Probes Plasmids Polymerase Chain Reaction Recombination, Genetic Restriction Mapping
Chemicals
DNA Transposable Elements DNA, Bacterial Oligonucleotide Probes DNA Nucleotidyltransferases Integrases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Storrs M J
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Poyart-Salmeron C
Trieu-Cuot P
Courvalin P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.14.4347-4352.1991
Published
1991-07-00
Pages
4347-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208095
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.173.14.4347-4352.1991 PMC Full Text View journal details
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