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PMID: 16525894 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An auxin-inducible F-box protein CEGENDUO negatively regulates auxin-mediated lateral root formation in Arabidopsis.

Plant molecular biology ·Vol. 60 ·No. 4 ·2006-03-00 ·Pages 599-615

Dong L, Wang L, Zhang Y, Zhang Y, Deng X, Xue Y

Abstract

Previously, we characterized 92 Arabidopsis genes (AtSFLs) similar to the S-locus F-box genes involved in S-RNase-based self-incompatibility and found that they likely play diverse roles in Arabidopsis. In this study, we investigated the role of one of these genes, CEGENDUO (CEG, AtSFL61), in the lateral root formation. A T-DNA insertion in CEG led to an increased lateral root production, which was complemented by transformation of the wild-type gene. Its downregulation by RNAi also produced more lateral roots in transformed Arabidopsis plants whereas its overexpression generated less lateral roots compared to wild-type, indicating that CEG acts as a negative regulator for the lateral root formation. It was found that CEG was expressed abundantly in vascular tissues of the primary root, but not in newly formed lateral root primordia and the root meristem, and induced by exogenous auxin NAA (alpha-naphthalene acetic acid). In addition, the ceg mutant was hyposensitive to NAA, IAA (indole-3-acetic acid) and 2,4-D (2,4-dichlorophenoxyacetic acid), as well as the auxin transport inhibitor TIBA (3,3,5-triiodobenzoic acid), showing that CEG is an auxin-inducible gene. Taken together, our results show that CEG is a novel F-box protein negatively regulating the auxin-mediated lateral root formation in Arabidopsis.

MeSH Terms
Arabidopsis/cytology,drug effects,metabolism Arabidopsis Proteins/genetics,metabolism Biological Transport/drug effects F-Box Proteins/genetics,metabolism Gene Expression Gene Expression Profiling Gene Expression Regulation, Plant/drug effects Genes, Plant/genetics Genetic Complementation Test Indoleacetic Acids/pharmacology Mutation/genetics Phenotype Plant Roots/cytology,drug effects,growth & development Plants, Genetically Modified/genetics RNA Interference RNA, Messenger/genetics,metabolism Repressor Proteins/metabolism Triiodobenzoic Acids/pharmacology
Chemicals
Arabidopsis Proteins CEGENDUO protein, Arabidopsis F-Box Proteins Indoleacetic Acids RNA, Messenger Repressor Proteins Triiodobenzoic Acids 2,3,5-triiodobenzoic acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dong Li
Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Centre for Plant Gene Research, Beijing 100080, China.
Wang Lei
Zhang Yu'e
Zhang Yansheng
Deng Xingwang
Xue Yongbiao
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-005-5257-5
Published
2006-03-00
Pages
599-615
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/s11103-005-5257-5 View journal details
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