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PMID: 16531490 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The U-box protein CMPG1 is required for efficient activation of defense mechanisms triggered by multiple resistance genes in tobacco and tomato.

The Plant cell ·Vol. 18 ·No. 4 ·2006-04-00 ·Pages 1067-83

González-Lamothe R, Tsitsigiannis DI, Ludwig AA, Panicot M, Shirasu K, Jones JD

Abstract

We previously identified three Avr9/Cf-9 Rapidly Elicited (ACRE) genes essential for Cf-9- and Cf-4-dependent hypersensitive response (HR) production in Nicotiana benthamiana. Two of them encode putative E3 ubiquitin ligase components. This led us to investigate other ACRE genes associated with the ubiquitination pathway. ACRE74 encodes a U-box E3 ligase homolog, highly related to parsley (Petroselinum crispum) CMPG1 and Arabidopsis thaliana PLANT U-BOX20 (PUB20) and PUB21 proteins, and was called Nt CMPG1. Transcript levels of Nt CMPG1 and the homologous tomato (Solanum lycopersicum) Cmpg1 are induced in Cf9 tobacco (Nicotiana tabacum) and Cf9 tomato after Avr9 elicitation. Tobacco CMPG1 possesses in vitro E3 ligase activity. N. benthamiana plants silenced for Nt CMPG1 show reduced HR after Cf-9/Avr9 elicitation, while overexpression of Nt CMPG1 induces a stronger HR in Cf9 tobacco plants after Avr9 infiltration. In tomato, silencing of Cmpg1 decreased resistance to Cladosporium fulvum. Overexpression of epitope-tagged tobacco CMPG1 mutated in the U-box domain confers a dominant-negative phenotype. We also show that Nt CMPG1 is involved in the Pto/AvrPto and Inf1 responses. In summary, we show that the E3 ligase Nt CMPG1 is essential for plant defense and disease resistance.

MeSH Terms
Amino Acid Sequence Base Sequence Consensus Sequence DNA Primers Genes, Dominant Immunity, Innate/genetics Lycopersicon esculentum/enzymology,genetics,physiology Molecular Sequence Data Mutation Plant Diseases Plant Proteins/metabolism Sequence Alignment Sequence Homology, Amino Acid Tobacco/enzymology,genetics,physiology Ubiquitin/metabolism Ubiquitin-Protein Ligases/genetics,metabolism
Chemicals
DNA Primers Plant Proteins Ubiquitin Ubiquitin-Protein Ligases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
González-Lamothe Rocío
Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, UK.
Tsitsigiannis Dimitrios I
Ludwig Andrea A
Panicot Mireia
Shirasu Ken
Jones Jonathan D G
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.106.040998
Published
2006-04-00
Epub
2006-00-10
Pages
1067-83
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1425846
Subset
IM
Databases
GENBANK
AAG51307, AAK69402, AAP03884, DQ118759
RefSeq
NP_198565
External Links
PubMed source DOI: 10.1105/tpc.106.040998 PMC Full Text View journal details
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