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PMID: 1653225 Published · ppublish English Journal Article

Nucleotide sequence of the gene (ard) encoding the antirestriction protein of plasmid colIb-P9.

Journal of bacteriology ·Vol. 173 ·No. 18 ·1991-09-00 ·Pages 5887-92

Delver EP, Kotova VU, Zavilgelsky GB, Belogurov AA

Abstract

The IncI1 plasmid ColIb-P9 was found to encode an antirestriction function. The relevant gene, ard (alleviation of restriction of DNA), maps about 5 kb from the origin of transfer, in the region transferred early during bacterial conjugation. Ard inhibits both restriction and modification by each of the four type I systems of Escherichia coli tested, but it had no effect on restriction by either EcoRI, a type II system, or EcoP1, a type III system. The nucleotide sequence of the ColIb ard gene was determined; the predicted molecular weight of the Ard polypeptide is 19,193. The proposed polypeptide chain contains an excess of 25 negatively charged amino acids, suggesting that its overall character is very acidic. Deletion analysis of the gene revealed that the Ard protein contained a distinct functional domain located in the COOH-terminal half of the polypeptide. We suggest that the biological role of the ColIb Ard protein is associated with overcoming host-controlled restriction during bacterial conjugation.

Related Genes
ard
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular Codon DNA Modification Methylases/metabolism DNA Restriction Enzymes/metabolism DNA, Bacterial/genetics Genes, Bacterial Molecular Sequence Data Plasmids Restriction Mapping Viral Proteins/genetics
Chemicals
Bacterial Proteins Codon DNA, Bacterial Viral Proteins anti-restriction proteins DNA Modification Methylases DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Delver E P
Department of Biotechnology, USSR Cardiology Research Center, Moscow.
Kotova V U
Zavilgelsky G B
Belogurov A A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.18.5887-5892.1991
Published
1991-09-00
Pages
5887-92
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208323
Subset
IM
Databases
GENBANK
M64126, M64127, M64128, M64129, M74923, S52988, S52991, S53819, S53821, S54509
External Links
PubMed source DOI: 10.1128/jb.173.18.5887-5892.1991 PMC Full Text View journal details
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