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PMID: 1654444 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration.

Journal of virology ·Vol. 65 ·No. 10 ·1991-10-00 ·Pages 5348-56

Rauh I, Mettenleiter TC

Abstract

Pseudorabies virus (PrV) glycoproteins gII and gp50 are major constituents of the viral envelope and targets of neutralizing monoclonal antibodies. Both are homologs of essential glycoproteins found in herpes simplex virus, gB (gII) and gD (gp50). We recently isolated a gII-negative PrV deletion mutant on complementing cell lines and established the essential character of gII for PrV replication (I. Rauh, F. Weiland, F. Fehler, G. Keil, and T.C. Mettenleiter, J. Virol. 65: 621-631, 1991). In this report, we describe the isolation of a gp50-negative PrV mutant after constructing cell lines that constitutively express gp50 and phenotypically complement the gp50 defect. Analysis of the gp50- mutant proved that gp50 is essential for PrV replication. Further studies showed that both gII and gp50 are required for viral penetration into target cells. The penetration defect in the gII and gp50 deletion mutants could be overcome by experimental polyethylene glycol-induced membrane fusion. Surprisingly, whereas gII proved to be essential for both penetration and cell-cell spread of the virus, gp50 was required only for penetration and appeared dispensable for direct cell-cell spread.

MeSH Terms
Animals Blotting, Southern Cell Line DNA, Viral/genetics,isolation & purification Genes, Viral Herpesvirus 1, Suid/genetics,physiology Mutagenesis Plasmids Restriction Mapping Viral Envelope Proteins/genetics,metabolism Viral Proteins/biosynthesis,genetics Virion/genetics,physiology Virus Replication
Chemicals
DNA, Viral Viral Envelope Proteins Viral Proteins glycoprotein gII, pseudorabies virus pseudorabies virus glycoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rauh I
Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.
Mettenleiter T C
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.10.5348-5356.1991
Published
1991-10-00
Pages
5348-56
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249015
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.10.5348-5356.1991 PMC Full Text View journal details
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