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PMID: 1655419 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The role of cyclins in the maturation of Patella vulgata oocytes.

The EMBO journal ·Vol. 10 ·No. 11 ·1991-11-00 ·Pages 3343-9

van Loon AE, Colas P, Goedemans HJ, Néant I, Dalbon P, Guerrier P

Abstract

We have cloned and sequenced the cDNAs encoding Patella vulgata cyclins A and B. The cDNA clones contain an open reading frame of 426 and 408 amino acids respectively, which present similarity with cyclins from other species. Cyclin A and B RNAs are present as polyadenylated and non-polyadenylated RNA in prophase oocytes and are completely polyadenylated in metaphase I. During the first cleavages after fertilization the level of cyclin A and B mRNAs is high and drops when the free swimming stage is reached. Using p13suc1-Sepharose bead precipitation we demonstrate that cyclin synthesis is triggered during maturation and that inhibition of protein synthesis makes the cyclins disappear rapidly from the metaphase I oocytes, which shift to interphase condition. By microinjecting antisense oligonucleotides into metaphase I oocytes, we demonstrate that in vivo ablation of cyclin A and B messengers together gives the same result, whereas microinjection of only one oligonucleotide does not show any effect.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cyclins/physiology DNA/genetics Humans Maturation-Promoting Factor/metabolism Metaphase Microinjections Molecular Sequence Data Mollusca/physiology Oligonucleotides, Antisense/metabolism Ovum/growth & development Precipitin Tests RNA, Messenger/metabolism Ribonuclease H Species Specificity
Chemicals
Cyclins Oligonucleotides, Antisense RNA, Messenger DNA Maturation-Promoting Factor Ribonuclease H
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
van Loon A E
Ecole Normale Superieure, Laboratoire de Biologie Cellulaire et Moleculaire, Lyon, France.
Colas P
Goedemans H J
Néant I
Dalbon P
Guerrier P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-11-00
Pages
3343-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453061
Subset
IM
Databases
GENBANK
X58357, X58358
External Links
PubMed source PMC Full Text View journal details
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