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PMID: 1673925 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

LFB3, a heterodimer-forming homeoprotein of the LFB1 family, is expressed in specialized epithelia.

The EMBO journal ·Vol. 10 ·No. 6 ·1991-06-00 ·Pages 1435-43

De Simone V, De Magistris L, Lazzaro D, Gerstner J, Monaci P, Nicosia A, Cortese R

Abstract

We have cloned and characterized a mouse cDNA coding for LFB3, a DNA binding protein containing an extra-large homeodomain. The first 315 amino acids of LFB3 are highly homologous to the DNA binding domain of LFB1, a regulatory protein involved in the expression of several liver-specific genes. LFB3 is a transcriptional activator which binds to DNA as a dimer and forms heterodimers with LFB1 both in vitro and in vivo. However, LFB3 expression seems not to be directly correlated with the liver-specific phenotype, since it is detected in dedifferentiated hepatoma cell lines which express neither LFB1 nor several liver-specific genes. LFB3 expression starts before that of LFB1 during mouse and rat development, and is strongly increased upon retinoic acid induced differentiation of F9 embryonic carcinoma cells. LFB3 and LFB1 are expressed in the epithelial component of many organs of endodermal and mesodermal origin, suggesting that they may play a more general role associated with the differentiation of specialized epithelia.

MeSH Terms
Amino Acid Sequence Animals Cell Line Cloning, Molecular DNA-Binding Proteins/genetics,metabolism Embryo, Mammalian/physiology Epithelium/physiology Gene Expression Gene Expression Regulation Genes, Homeobox Hepatocyte Nuclear Factor 1-beta Humans In Vitro Techniques Liver/embryology,physiology Macromolecular Substances Mice Molecular Sequence Data Multigene Family Nucleic Acid Hybridization Promoter Regions, Genetic Recombinant Proteins/metabolism Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
DNA-Binding Proteins HNF1B protein, human Hnf1b protein, mouse Macromolecular Substances Recombinant Proteins Transcription Factors Hepatocyte Nuclear Factor 1-beta
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
De Simone V
European Molecular Biology Laboratory, Heidelberg, FRG.
De Magistris L
Lazzaro D
Gerstner J
Monaci P
Nicosia A
Cortese R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-06-00
Pages
1435-43
Language
English
Region
England
NLM ID
8208664
PMCID
PMC452805
Subset
IM
Databases
GENBANK
M55400, M55401, M55402, M65224, S60919, S60920, X55842, X59082, X59870, X59871
External Links
PubMed source PMC Full Text View journal details
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