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PMID: 16818719 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

COPII-Golgi protein interactions regulate COPII coat assembly and Golgi size.

The Journal of cell biology ·Vol. 174 ·No. 1 ·2006-07-03 ·Pages 53-63

Guo Y, Linstedt AD

Abstract

Under experimental conditions, the Golgi apparatus can undergo de novo biogenesis from the endoplasmic reticulum (ER), involving a rapid phase of growth followed by a return to steady state, but the mechanisms that control growth are unknown. Quantification of coat protein complex (COP) II assembly revealed a dramatic up-regulation at exit sites driven by increased levels of Golgi proteins in the ER. Analysis in a permeabilized cell assay indicated that up-regulation of COPII assembly occurred in the absence GTP hydrolysis and any cytosolic factors other than the COPII prebudding complex Sar1p-Sec23p-Sec24p. Remarkably, acting via a direct interaction with Sar1p, increased expression of the Golgi enzyme N-acetylgalactosaminyl transferase-2 induced increased COPII assembly on the ER and an overall increase in the size of the Golgi apparatus. These results suggest that direct interactions between Golgi proteins exiting the ER and COPII components regulate ER exit, providing a variable exit rate mechanism that ensures homeostasis of the Golgi apparatus.

MeSH Terms
Animals COP-Coated Vesicles/metabolism Cell Line Cells, Cultured Endoplasmic Reticulum/metabolism Golgi Apparatus/metabolism HeLa Cells Homeostasis Humans Models, Biological N-Acetylgalactosaminyltransferases/metabolism Rats
Chemicals
N-Acetylgalactosaminyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Guo Yusong
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Linstedt Adam D
References (49)
49 references, click to expand
  1. Decoding of sorting signals by coatomer through a GTPase switch in the COPI coat complex.
    Cell. 2000 Mar 17;100(6):671-9 PMID: 10761932
  2. Nm23H2 facilitates coat protein complex II assembly and endoplasmic reticulum export in mammalian cells.
    Mol Biol Cell. 2005 Feb;16(2):835-48 PMID: 15591128
  3. Dynamics of transitional endoplasmic reticulum sites in vertebrate cells.
    Mol Biol Cell. 2000 Sep;11(9):3013-30 PMID: 10982397
  4. The Sar1 GTPase coordinates biosynthetic cargo selection with endoplasmic reticulum export site assembly.
    J Cell Biol. 2001 Jan 8;152(1):213-29 PMID: 11149932
  5. Golgi complex reorganization during muscle differentiation: visualization in living cells and mechanism.
    Mol Biol Cell. 2001 Apr;12(4):795-808 PMID: 11294887
  6. Dynamics of the COPII coat with GTP and stable analogues.
    Nat Cell Biol. 2001 Jun;3(6):531-7 PMID: 11389436
  7. Mammalian cell morphology and endocytic membrane homeostasis require enzymatically active phosphoinositide 5-kinase PIKfyve.
    J Biol Chem. 2001 Jul 13;276(28):26141-7 PMID: 11285266
  8. Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130.
    J Cell Biol. 2001 Oct 15;155(2):227-38 PMID: 11591729
  9. Maintenance of Golgi structure and function depends on the integrity of ER export.
    J Cell Biol. 2001 Nov 12;155(4):557-70 PMID: 11706049
  10. The delta subunit of AP-3 is required for efficient transport of VSV-G from the trans-Golgi network to the cell surface.
    Proc Natl Acad Sci U S A. 2002 May 14;99(10):6755-60 PMID: 11997454
  11. Cargo selection in vesicular transport: the making and breaking of a coat.
    Traffic. 2002 Aug;3(8):537-46 PMID: 12121417
  12. Cycling of early Golgi proteins via the cell surface and endosomes upon lumenal pH disruption.
    Traffic. 2002 Sep;3(9):641-53 PMID: 12191016
  13. Structural organization of the endoplasmic reticulum.
    EMBO Rep. 2002 Oct;3(10):944-50 PMID: 12370207
  14. Hepatocyte growth factor switches orientation of polarity and mode of movement during morphogenesis of multicellular epithelial structures.
    Mol Biol Cell. 2003 Feb;14(2):748-63 PMID: 12589067
  15. Cell-cycle-specific Golgi fragmentation: how and why?
    Curr Opin Cell Biol. 2003 Aug;15(4):462-7 PMID: 12892787
  16. SNARE selectivity of the COPII coat.
    Cell. 2003 Aug 22;114(4):483-95 PMID: 12941276
  17. Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein.
    Nature. 1992 Nov 26;360(6402):350-2 PMID: 1448151
  18. Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF.
    Nature. 1992 Nov 26;360(6402):352-4 PMID: 1448152
  19. Requirement for a GTPase-activating protein in vesicle budding from the endoplasmic reticulum.
    Science. 1993 Mar 5;259(5100):1466-8 PMID: 8451644
  20. Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa.
    Mol Biol Cell. 1993 Jul;4(7):679-93 PMID: 7691276
  21. "BFA bodies": a subcompartment of the endoplasmic reticulum.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11089-93 PMID: 8248213
  22. COPI- and COPII-coated vesicles bud directly from the endoplasmic reticulum in yeast.
    Cell. 1995 Dec 29;83(7):1183-96 PMID: 8548805
  23. Regulation of constitutive exocytic transport by membrane receptors. A biochemical and morphometric study.
    J Biol Chem. 1996 Feb 16;271(7):3523-33 PMID: 8631957
  24. Effects of overexpression of the transferrin receptor on the rates of transferrin recycling and uptake of non-transferrin-bound iron.
    Eur J Biochem. 1996 Jun 1;238(2):463-9 PMID: 8681959
  25. Mannose 6-phosphate receptors regulate the formation of clathrin-coated vesicles in the TGN.
    J Cell Biol. 1997 Apr 21;137(2):335-45 PMID: 9128246
  26. Ultrastructural changes of the myocardium in the embryonic rat heart.
    Anat Rec. 1997 Jun;248(2):233-41 PMID: 9185989
  27. Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis.
    Nat Struct Mol Biol. 2005 Feb;12(2):167-74 PMID: 15665868
  28. Secretory cargo regulates the turnover of COPII subunits at single ER exit sites.
    Curr Biol. 2006 Jan 24;16(2):173-9 PMID: 16431369
  29. Biogenesis of tubular ER-to-Golgi transport intermediates.
    Mol Biol Cell. 2006 Feb;17(2):723-37 PMID: 16314391
  30. Multiple cargo binding sites on the COPII subunit Sec24p ensure capture of diverse membrane proteins into transport vesicles.
    Cell. 2003 Aug 22;114(4):497-509 PMID: 12941277
  31. Endoplasmic reticulum export of glycosyltransferases depends on interaction of a cytoplasmic dibasic motif with Sar1.
    Mol Biol Cell. 2003 Sep;14(9):3753-66 PMID: 12972562
  32. Capacity of the golgi apparatus for biogenesis from the endoplasmic reticulum.
    Mol Biol Cell. 2003 Dec;14(12):5011-8 PMID: 14565973
  33. The mechanisms of vesicle budding and fusion.
    Cell. 2004 Jan 23;116(2):153-66 PMID: 14744428
  34. GTP/GDP exchange by Sec12p enables COPII vesicle bud formation on synthetic liposomes.
    EMBO J. 2004 Oct 27;23(21):4146-55 PMID: 15457212
  35. Transferrin receptors promote the formation of clathrin lattices.
    Cell. 1991 May 17;65(4):621-32 PMID: 1903330
  36. Sequence and overexpression of GPP130/GIMPc: evidence for saturable pH-sensitive targeting of a type II early Golgi membrane protein.
    Mol Biol Cell. 1997 Jun;8(6):1073-87 PMID: 9201717
  37. The yeast spindle pole body is assembled around a central crystal of Spc42p.
    Cell. 1997 Jun 27;89(7):1077-86 PMID: 9215630
  38. A di-acidic signal required for selective export from the endoplasmic reticulum.
    Science. 1997 Jul 25;277(5325):556-8 PMID: 9228004
  39. COPII-cargo interactions direct protein sorting into ER-derived transport vesicles.
    Nature. 1998 Jan 8;391(6663):187-90 PMID: 9428766
  40. gp25L/emp24/p24 protein family members of the cis-Golgi network bind both COP I and II coatomer.
    J Cell Biol. 1998 Feb 23;140(4):751-65 PMID: 9472029
  41. Cargo selection by the COPII budding machinery during export from the ER.
    J Cell Biol. 1998 Apr 6;141(1):61-70 PMID: 9531548
  42. COPII-coated vesicle formation reconstituted with purified coat proteins and chemically defined liposomes.
    Cell. 1998 Apr 17;93(2):263-75 PMID: 9568718
  43. Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs.
    Science. 1998 Jul 31;281(5377):698-700 PMID: 9685263
  44. Cargo can modulate COPII vesicle formation from the endoplasmic reticulum.
    J Biol Chem. 1999 Feb 12;274(7):4389-99 PMID: 9933643
  45. A primer on vesicle budding.
    Cell. 1999 Apr 16;97(2):145-8 PMID: 10219233
  46. Osmotically induced cell volume changes alter anterograde and retrograde transport, Golgi structure, and COPI dissociation.
    Mol Biol Cell. 1999 May;10(5):1445-62 PMID: 10233155
  47. Spc29p is a component of the Spc110p subcomplex and is essential for spindle pole body duplication.
    Proc Natl Acad Sci U S A. 1999 May 25;96(11):6205-10 PMID: 10339566
  48. Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments.
    Nat Cell Biol. 2004 Nov;6(11):1071-81 PMID: 15502824
  49. Potential role for protein kinases in regulation of bidirectional endoplasmic reticulum-to-Golgi transport revealed by protein kinase inhibitor H89.
    Mol Biol Cell. 2000 Aug;11(8):2577-90 PMID: 10930455
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200604058
Published
2006-07-03
Pages
53-63
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064162
Subset
IM
Grants
NIGMS NIH HHS · R01 GM056779 · United States
NIGMS NIH HHS · GM-56779 · United States
External Links
PubMed source DOI: 10.1083/jcb.200604058 PMC Full Text View journal details
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