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PMID: 9128246 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mannose 6-phosphate receptors regulate the formation of clathrin-coated vesicles in the TGN.

The Journal of cell biology ·Vol. 137 ·No. 2 ·1997-04-21 ·Pages 335-45

Le Borgne R, Hoflack B

Abstract

The transport of the two mannose 6-phosphate receptors (MPRs) from the secretory pathway to the endocytic pathway is mediated by carrier vesicles coated with the AP-1 Golgi-specific assembly protein and clathrin. Using an in vitro assay that reconstitutes the ARF-1-dependent translocation of cytosolic AP-1 onto membranes of the TGN, we have previously reported that the MPRs are key components for the efficient recruitment of AP-1 (Le Borgne, R., G. Griffiths, and B. Hoflack. 1996. J. Biol. Chem. 271:2162-2170). Using a polyclonal antibody against the mouse gamma-adaptin, we have now examined the steady state distribution of AP-1 after subcellular fractionation of mouse fibroblasts lacking both MPRs or reexpressing physiological levels of either MPR. We report that the amount of AP-1 bound to membranes and associated with clathrin-coated vesicles depends on the expression level of the MPRs and on the integrity of their cytoplasmic domains. Thus, these results indicate that the concentration of the MPRs, i.e., the major transmembrane proteins sorted toward the endosomes, determines the number of clathrin-coated vesicles formed in the TGN.

MeSH Terms
Adaptor Protein Complex alpha Subunits Adaptor Protein Complex gamma Subunits Adaptor Proteins, Vesicular Transport Amino Acid Sequence Animals Biological Transport Biomarkers/analysis Cell Fractionation Cells, Cultured Clathrin/analysis Coated Vesicles/metabolism Cytoplasm Endocytosis/physiology Fibroblasts Golgi Apparatus/chemistry,metabolism Intracellular Membranes/chemistry Membrane Proteins/analysis Mice Molecular Sequence Data Mutation Receptor, IGF Type 2/genetics,physiology Receptors, Transferrin/metabolism
Chemicals
Adaptor Protein Complex alpha Subunits Adaptor Protein Complex gamma Subunits Adaptor Proteins, Vesicular Transport Biomarkers Clathrin Membrane Proteins Receptor, IGF Type 2 Receptors, Transferrin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Le Borgne R
European Molecular Biology Laboratory, Heidelberg, Germany.
Hoflack B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.137.2.335
Published
1997-04-21
Pages
335-45
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2139777
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.137.2.335 PMC Full Text View journal details
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