Home LiteratureArticle Details
PMID: 1688886 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Peptide growth factors can provoke "fetal" contractile protein gene expression in rat cardiac myocytes.

The Journal of clinical investigation ·Vol. 85 ·No. 2 ·1990-02-00 ·Pages 507-14

Parker TG, Packer SE, Schneider MD

Abstract

Cardiac-specific gene expression is intricately regulated in response to developmental, hormonal, and hemodynamic stimuli. To test whether cardiac muscle might be a target for regulation by peptide growth factors, the effect of three growth factors on the actin and myosin gene families was investigated by Northern blot analysis in cultured neonatal rat cardiac myocytes. Transforming growth factor-beta 1 (TGF beta 1, 1 ng/ml) and basic fibroblast growth factor (FGF, 25 ng/ml) elicited changes corresponding to those induced by hemodynamic load. The "fetal" beta-myosin heavy chain (MHC) was up-regulated about four-fold, whereas the "adult" alpha MHC was inhibited greater than 50-60%; expression of alpha-skeletal actin increased approximately two-fold, with little or no change in alpha-cardiac actin. Thus, peptide growth factors alter the program of differentiated gene expression in cardiac myocytes, and are sufficient to provoke fetal contractile protein gene expression, characteristic of pressure-overload hypertrophy. Acidic FGF (25 ng/ml) produced seven- to eightfold reciprocal changes in MHC expression but, unlike either TGF-beta 1 or basic FGF, inhibited both striated alpha-actin genes by 70-90%. Expression of vascular smooth muscle alpha-actin, the earliest alpha-actin induced during cardiac myogenesis, was increased by all three growth factors. Thus, three alpha-actin genes demonstrate distinct responses to acidic vs. basic FGF.

MeSH Terms
Actins/genetics Animals Animals, Newborn/metabolism Cardiomegaly/etiology Cell Differentiation Cells, Cultured Fibroblast Growth Factors/pharmacology Gene Expression Regulation/drug effects Major Histocompatibility Complex Myocardium/metabolism Myosins/genetics RNA/analysis Rats Rats, Inbred Strains Transforming Growth Factors/pharmacology
Chemicals
Actins Fibroblast Growth Factors RNA Transforming Growth Factors Myosins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Parker T G
Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.
Packer S E
Schneider M D
References (56)
56 references, click to expand
  1. Mitogens and oncogenes can block the induction of specific voltage-gated ion channels.
    Science. 1987 May 1;236(4801):570-3 PMID: 2437651
  2. Control by fibroblast growth factor of differentiation in the BC3H1 muscle cell line.
    J Cell Biol. 1985 May;100(5):1540-7 PMID: 3988800
  3. Transformation by activated ras or fos prevents myogenesis by inhibiting expression of MyoD1.
    Cell. 1989 Aug 25;58(4):659-67 PMID: 2548731
  4. Ca2+ and Na+ currents in developing skeletal myoblasts are expressed in a sequential program: reversible suppression by transforming growth factor beta-1, an inhibitor of the myogenic pathway.
    J Neurosci. 1989 Oct;9(10):3443-53 PMID: 2552033
  5. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.
    Cell. 1989 Feb 24;56(4):607-17 PMID: 2537150
  6. Inhibition of myogenic differentiation by the H-ras oncogene is associated with the down regulation of the MyoD1 gene.
    Oncogene. 1989 Apr;4(4):473-81 PMID: 2654809
  7. Expression of transforming growth factor-beta 1 in specific cells and tissues of adult and neonatal mice.
    J Cell Biol. 1989 Feb;108(2):661-9 PMID: 2645303
  8. Identification of a myocyte nuclear factor that binds to the muscle-specific enhancer of the mouse muscle creatine kinase gene.
    Mol Cell Biol. 1989 Jun;9(6):2627-40 PMID: 2761542
  9. Epithelial-mesenchymal cell transformation in the embryonic heart can be mediated, in part, by transforming growth factor beta.
    Dev Biol. 1989 Aug;134(2):392-401 PMID: 2744239
  10. Decrease in transforming growth factor-beta binding and action during differentiation in muscle cells.
    J Biol Chem. 1988 Mar 15;263(8):4029-32 PMID: 2894374
  11. Sequential activation of alpha-actin genes during avian cardiogenesis: vascular smooth muscle alpha-actin gene transcripts mark the onset of cardiomyocyte differentiation.
    J Cell Biol. 1988 Dec;107(6 Pt 2):2575-86 PMID: 3204121
  12. Terminally differentiated neonatal rat myocardial cells proliferate and maintain specific differentiated functions following expression of SV40 large T antigen.
    J Biol Chem. 1988 Dec 15;263(35):19132-6 PMID: 3198615
  13. Differentiation of cardiac myocytes after mitogen withdrawal exhibits three sequential states of the ventricular growth response.
    J Cell Biol. 1988 Nov;107(5):1911-8 PMID: 3182941
  14. The nucleotide sequence of a rat vascular smooth muscle alpha-actin cDNA.
    Nucleic Acids Res. 1988 May 11;16(9):4167 PMID: 3375082
  15. Acidic and basic fibroblast growth factors promote stable neurite outgrowth and neuronal differentiation in cultures of PC12 cells.
    J Neurosci. 1987 Nov;7(11):3639-53 PMID: 3316527
  16. Synergistic induction of mesoderm by FGF and TGF-beta and the identification of an mRNA coding for FGF in the early Xenopus embryo.
    Cell. 1987 Dec 4;51(5):869-77 PMID: 3479265
  17. Inhibition of myogenic differentiation by fibroblast growth factor or type beta transforming growth factor does not require persistent c-myc expression.
    Dev Biol. 1987 Oct;123(2):500-7 PMID: 3477514
  18. Regulation of myogenic differentiation by type beta transforming growth factor.
    J Cell Biol. 1986 Nov;103(5):1799-805 PMID: 3465734
  19. Transforming growth factor-beta. A very potent inhibitor of myoblast differentiation, identical to the differentiation inhibitor secreted by Buffalo rat liver cells.
    J Biol Chem. 1986 Dec 15;261(35):16509-13 PMID: 3465726
  20. Expression of a single transfected cDNA converts fibroblasts to myoblasts.
    Cell. 1987 Dec 24;51(6):987-1000 PMID: 3690668
  21. Growth factor control of skeletal muscle differentiation: commitment to terminal differentiation occurs in G1 phase and is repressed by fibroblast growth factor.
    J Cell Biol. 1987 Aug;105(2):949-56 PMID: 3624313
  22. Mesoderm induction in early Xenopus embryos by heparin-binding growth factors.
    Nature. 1987 Mar 12-18;326(6109):197-200 PMID: 3821895
  23. Formation of binucleated myocardial cells in the neonatal rat. An index for growth hypertrophy.
    Lab Invest. 1984 May;50(5):571-7 PMID: 6232423
  24. Reversibility of muscle differentiation in the absence of commitment: analysis of a myogenic cell line temperature-sensitive for commitment.
    Cell. 1983 Aug;34(1):281-93 PMID: 6683997
  25. Myosin isoenzymic distribution correlates with speed of myocardial contraction.
    J Mol Cell Cardiol. 1981 Dec;13(12):1071-5 PMID: 7328666
  26. Myosin isoenzyme redistribution in chronic heart overload.
    Nature. 1979 Nov 1;282(5734):105-7 PMID: 91973
  27. Molecular signals for initiating protein synthesis in organ hypertrophy.
    Proc Natl Acad Sci U S A. 1979 May;76(5):2455-9 PMID: 156367
  28. Interrelations of the proliferation and differentiation processes during cardiact myogenesis and regeneration.
    Int Rev Cytol. 1977;51:186-273 PMID: 338537
  29. An improved procedure for serum creatine phosphokinase determination.
    J Lab Clin Med. 1967 Apr;69(4):696-705 PMID: 4381359
  30. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  31. Proto-oncogenes and cardiac hypertrophy.
    Annu Rev Physiol. 1989;51:189-202 PMID: 2469381
  32. A hemodynamic load in vivo induces cardiac expression of the cellular oncogene, c-myc.
    Biochem Biophys Res Commun. 1987 Sep 15;147(2):627-36 PMID: 2958008
  33. Induction of the skeletal alpha-actin gene in alpha 1-adrenoceptor-mediated hypertrophy of rat cardiac myocytes.
    J Clin Invest. 1987 Oct;80(4):1194-9 PMID: 2821075
  34. An activated c-Ha-ras allele blocks the induction of muscle-specific genes whose expression is contingent on mitogen withdrawal.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8956-60 PMID: 3122209
  35. Protooncogene induction and reprogramming of cardiac gene expression produced by pressure overload.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):339-43 PMID: 2963328
  36. Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8348-50 PMID: 3022291
  37. Myosin heavy chain messenger RNA and protein isoform transitions during cardiac hypertrophy. Interaction between hemodynamic and thyroid hormone-induced signals.
    J Clin Invest. 1987 Mar;79(3):970-7 PMID: 2950137
  38. Alpha-skeletal muscle actin mRNA's accumulate in hypertrophied adult rat hearts.
    Circ Res. 1986 Nov;59(5):551-5 PMID: 2948733
  39. Cell surface fibroblast growth factor and epidermal growth factor receptors are permanently lost during skeletal muscle terminal differentiation in culture.
    J Cell Biol. 1988 Aug;107(2):761-9 PMID: 2843547
  40. Expression of cellular oncogenes in the myocardium during the developmental stage and pressure-overloaded hypertrophy of the rat heart.
    Circ Res. 1988 Jun;62(6):1075-9 PMID: 2454761
  41. Transforming growth factor type beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro.
    Proc Natl Acad Sci U S A. 1986 Jun;83(12):4167-71 PMID: 2424019
  42. The oncogenic forms of N-ras or H-ras prevent skeletal myoblast differentiation.
    Mol Cell Biol. 1987 Jun;7(6):2104-11 PMID: 3600660
  43. A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program.
    Genes Dev. 1989 May;3(5):628-40 PMID: 2473006
  44. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  45. Control of myogenic differentiation by cellular oncogenes.
    Mol Neurobiol. 1988 Spring;2(1):1-39 PMID: 3077064
  46. Fibroblast growth factor and transforming growth factor beta repress transcription of the myogenic regulatory gene MyoD1.
    Mol Cell Biol. 1989 Aug;9(8):3576-9 PMID: 2797000
  47. Type beta transforming growth factor is an inhibitor of myogenic differentiation.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8206-10 PMID: 3022285
  48. Developmental and functional adaptation of contractile proteins in cardiac and skeletal muscles.
    Physiol Rev. 1986 Jul;66(3):710-71 PMID: 2942954
  49. Transforming growth factor beta-1 in acute myocardial infarction in rats.
    Growth Factors. 1988;1(1):91-9 PMID: 3078566
  50. Myogenic lineage determination and differentiation: evidence for a regulatory gene pathway.
    Cell. 1988 Jun 3;53(5):781-93 PMID: 3286015
  51. Acidic fibroblast growth factor mRNA is expressed by cardiac myocytes in culture and the protein is localized to the extracellular matrix.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2683-7 PMID: 2468157
  52. Inhibitory action of transforming growth factor beta on endothelial cells.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5600-4 PMID: 2441387
  53. Structural characterization and biological functions of fibroblast growth factor.
    Endocr Rev. 1987 May;8(2):95-114 PMID: 2440668
  54. Acidic and basic fibroblast growth factors stimulate tyrosine kinase activity in vivo.
    J Biol Chem. 1988 Jan 15;263(2):988-93 PMID: 2447083
  55. Effects of thyroid hormone on alpha-actin and myosin heavy chain gene expression in cardiac and skeletal muscles of the rat: measurement of mRNA content using synthetic oligonucleotide probes.
    Circ Res. 1986 Aug;59(2):194-201 PMID: 3742743
  56. Transcriptional and posttranscriptional control of c-myc during myogenesis: its mRNA remains inducible in differentiated cells and does not suppress the differentiated phenotype.
    Mol Cell Biol. 1986 May;6(5):1412-21 PMID: 2431278
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114466
Published
1990-02-00
Pages
507-14
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC296452
Subset
IM
Grants
NHLBI NIH HHS · HL-39141 · United States
External Links
PubMed source DOI: 10.1172/JCI114466 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2