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PMID: 17070797 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

lin-35/Rb and the CoREST ortholog spr-1 coordinately regulate vulval morphogenesis and gonad development in C. elegans.

Developmental biology ·Vol. 302 ·No. 2 ·2007-02-15 ·Pages 448-62

Bender AM, Kirienko NV, Olson SK, Esko JD, Fay DS

Abstract

Using a genetic screen to identify genes that carry out redundant functions during development with lin-35/Rb, the C. elegans Retinoblastoma family ortholog, we have identified a mutation in spr-1. spr-1 encodes the C. elegans ortholog of human CoREST, a protein containing Myb-like SANT and ELM2 domains, which functions as part of a transcriptional regulatory complex. CoREST recruits mediators of transcriptional repression, including histone deacetylase, and demethylase, and interacts with the tumor suppression protein REST. spr-1/CoREST was previously shown in C. elegans to suppress defects associated with loss of the presenilin sel-12, which functions in the proteolytic processing of LIN-12/Notch. Here we show that lin-35 and spr-1 coordinately regulate several developmental processes in C. elegans including the ingression of vulval cells as well as germline proliferation. We also show that loss of lin-35 and spr-1 hypersensitizes animals to a reduction in LIN-12/Notch activity, leading to the generation of proximal germline tumors. This defect, which is observed in lin-35; spr-1; lin-12(RNAi) and lin-35; spr-1; hop-1(RNAi) triple mutants is likely due to a delay in the entry of germ cells into meiosis.

MeSH Terms
Animals Caenorhabditis elegans/growth & development,physiology Caenorhabditis elegans Proteins/genetics,physiology Cell Proliferation Chondroitin/biosynthesis Female Germ Cells/physiology Gonads/growth & development,physiology Larva Membrane Proteins/genetics,physiology Models, Animal Morphogenesis Mutation Neoplasms, Germ Cell and Embryonal/pathology Receptors, Notch/physiology Repressor Proteins/genetics,physiology Retinoblastoma Protein/genetics,physiology Signal Transduction Vulva/growth & development,physiology
Chemicals
Caenorhabditis elegans Proteins Lin-12 protein, C elegans Membrane Proteins Receptors, Notch Repressor Proteins Retinoblastoma Protein lin-35 protein, C elegans Chondroitin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bender Aaron M
University of Wyoming, College of Agriculture, Department of Molecular Biology, Department 3944, 1000 E. University Avenue, Laramie, WY 82071, USA.
Kirienko Natalia V
Olson Sara K
Esko Jeffery D
Fay David S
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Article Info
Journal
Developmental biology
Abbr.
Dev Biol
ISSN
0012-1606
DOI
10.1016/j.ydbio.2006.09.051
Published
2007-02-15
Epub
2006-00-05
Pages
448-62
Language
English
Region
United States
NLM ID
0372762
PMCID
PMC1933485
Subset
IM
Grants
NIGMS NIH HHS · GM066868 · United States
NIGMS NIH HHS · R01 GM066868-01A1 · United States
NIGMS NIH HHS · R01 GM033063 · United States
NIGMS NIH HHS · R01 GM033063-17 · United States
NIGMS NIH HHS · R01 GM066868 · United States
PHS HHS · G33063 · United States
NIGMS NIH HHS · R01 GM066868-02 · United States
External Links
PubMed source DOI: 10.1016/j.ydbio.2006.09.051 PMC Full Text View journal details
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