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PMID: 1707163 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA protein interactions at the interferon-responsive promoter elements: potential for an H-DNA conformation.

Nucleic acids research ·Vol. 19 ·No. 3 ·1991-02-11 ·Pages 517-24

Roy C, Lebleu B

Abstract

The regions of several genes (IFI-56K, HLA-A3, HLA-DR and 6-16) containing the (putative) ISRE (Interferon Stimulatable Response Element) were tested for their ability to be recognized by HeLa cells nuclear extract proteins. In a band shift assay, all probes yielded two B1 and B2 DNA-protein complexes of similar mobilities. Unexpectedly the titration of the B1 complex with a synthetic ISRE core (OL1), promoted the formation of B2. Both the probe and OL1 were recovered in B2. For each probe, the possibility of the part of the sequence involved in B1 complex to form a H-DNA structure with the part of the sequence involved in B2 exists. Such a structure was favored by the colinearity of the pairing regions and requires ATP. Although probes seemed to have a secondary structure, the formal existence of a H-DNA structure has not been demonstrated. Such a model could be extended to other interferon inducible gene promoters and may account for their binding properties and differential inducibility after 5' deletion or point mutations.

Related Genes
HLA-A3 HLA-DR IFI-56K
MeSH Terms
2',5'-Oligoadenylate Synthetase/genetics Adenosine Triphosphate/metabolism Base Sequence DNA/ultrastructure DNA Probes DNA-Binding Proteins/metabolism Gene Expression Regulation HLA-A3 Antigen/genetics HLA-DR Antigens/genetics Humans In Vitro Techniques Interferons/physiology Molecular Sequence Data Molecular Structure Nucleic Acid Conformation Oligonucleotides/chemistry Promoter Regions, Genetic Sequence Homology, Nucleic Acid
Chemicals
DNA Probes DNA-Binding Proteins HLA-A3 Antigen HLA-DR Antigens Oligonucleotides Adenosine Triphosphate DNA Interferons 2',5'-Oligoadenylate Synthetase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roy C
Laboratoire de Biochimie des Protéines, URA CNRS 1191, Université Montpellier II, France.
Lebleu B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.3.517
Published
1991-02-11
Pages
517-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC333642
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/19.3.517 PMC Full Text View journal details
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