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PMID: 1715324 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of M. tuberculosis.

Infection and immunity ·Vol. 59 ·No. 9 ·1991-09-00 ·Pages 3205-12

Content J, de la Cuvellerie A, De Wit L, Vincent-Levy-Frébault V, Ooms J, De Bruyn J

Abstract

A gene encoding the 33-kDa secreted protein of Mycobacterium tuberculosis (antigen 85-C) was isolated and sequenced. The corresponding DNA sequence contains a 1,020-bp coding region. The deduced amino acid sequence corresponds to a 340-residue protein consisting of a 46-amino-acid signal peptide and a 294-amino-acid mature protein. Comparison with previously described genes for the 30-kDa antigen (the alpha antigen of M. bovis BCG, also called antigen 85-B) and the 32-kDa antigens from M. bovis BCG and M. tuberculosis (antigens 85-A) indicates that the three genes share considerable sequence homology (70.8 to 77.5%) but may also code for distinctive epitopes. Strong differences among the three sequences are clearly visible upstream and downstream from the region coding for the mature proteins. The three genes have been detected in the genome of M. bovis BCG by Southern blot hybridization with three type-specific probes. Furthermore, hybridization of large DNA fragments (100 to 1,000 kbp) from M. tuberculosis separated by pulsed-field gel electrophoresis showed that the three genes coding for the antigen 85 complex are not clustered within the bacterial genome.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/genetics Base Sequence Blotting, Southern DNA, Bacterial/genetics Electrophoresis, Polyacrylamide Gel Epitopes/immunology Molecular Sequence Data Mycobacterium bovis/genetics,immunology Mycobacterium tuberculosis/genetics,immunology Nucleic Acid Hybridization Polymerase Chain Reaction Protein Sorting Signals/genetics Sequence Homology, Nucleic Acid
Chemicals
Antigens, Bacterial DNA, Bacterial Epitopes Protein Sorting Signals
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Content J
Department of Virology, Institut Pasteur du Brabant, Brussels, Belgium.
de la Cuvellerie A
De Wit L
Vincent-Levy-Frébault V
Ooms J
De Bruyn J
References (26)
26 references, click to expand
  1. Specific lymphoproliferation, gamma interferon production, and serum immunoglobulin G directed against a purified 32 kDa mycobacterial protein antigen (P32) in patients with active tuberculosis.
    Scand J Immunol. 1988 Feb;27(2):187-94 PMID: 3124263
  2. Characterization of fibronectin-binding antigens released by Mycobacterium tuberculosis and Mycobacterium bovis BCG.
    Infect Immun. 1988 Dec;56(12):3046-51 PMID: 3141278
  3. Dissection of Mycobacterium tuberculosis antigens using recombinant DNA.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2583-7 PMID: 2581251
  4. DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis.
    J Clin Microbiol. 1989 Dec;27(12):2823-6 PMID: 2574186
  5. Cloning, sequence determination, and expression of a 32-kilodalton-protein gene of Mycobacterium tuberculosis.
    Infect Immun. 1989 Oct;57(10):3123-30 PMID: 2506131
  6. The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.
    Nucleic Acids Res. 1988 May 25;16(10):4637-50 PMID: 3380691
  7. MPB59, a widely cross-reacting protein of Mycobacterium bovis BCG.
    Int Arch Allergy Appl Immunol. 1986;81(4):307-14 PMID: 3536756
  8. Cloning of Mycobacterium bovis BCG DNA and expression of antigens in Escherichia coli.
    Infect Immun. 1985 Dec;50(3):800-6 PMID: 3905615
  9. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
    DNA. 1985 Apr;4(2):165-70 PMID: 3996185
  10. Quantitative and qualitative studies on the major extracellular antigen of Mycobacterium tuberculosis H37Rv and Mycobacterium bovis BCG.
    Am Rev Respir Dis. 1990 Apr;141(4 Pt 1):830-8 PMID: 2109556
  11. Cloning and expression of the gene for the cross-reactive alpha antigen of Mycobacterium kansasii.
    Infect Immun. 1990 Feb;58(2):550-6 PMID: 2404875
  12. Nucleotide sequence of the 32 kDa-protein gene (antigen 85 A) of Mycobacterium bovis BCG.
    Nucleic Acids Res. 1990 Jul 11;18(13):3995 PMID: 2197602
  13. DNA recombination during PCR.
    Nucleic Acids Res. 1990 Apr 11;18(7):1687-91 PMID: 2186361
  14. Evidence for three separate genes encoding the proteins of the mycobacterial antigen 85 complex.
    Infect Immun. 1990 Jan;58(1):272-4 PMID: 2403534
  15. The antigens of Mycobacterium bovis, strain BCG, studied by crossed immunoelectrophoresis: a reference system.
    Scand J Immunol. 1980;12(3):249-63 PMID: 7008186
  16. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  17. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  18. A localization index for distinction between extracellular and intracellular antigens of Mycobacterium tuberculosis.
    J Gen Microbiol. 1991 Apr;137(4):875-84 PMID: 1906925
  19. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  20. Hybrid DNA artifact from PCR of closely related target sequences.
    Nucleic Acids Res. 1989 Jun 12;17(11):4409 PMID: 2740241
  21. Purification, characterization and identification of a 32 kDa protein antigen of Mycobacterium bovis BCG.
    Microb Pathog. 1987 May;2(5):351-66 PMID: 3148811
  22. Apple Macintosh programs for nucleic and protein sequence analyses.
    Nucleic Acids Res. 1988 Mar 11;16(5):1837-46 PMID: 2832832
  23. Cloning and expression of the Mycobacterium bovis BCG gene for extracellular alpha antigen.
    J Bacteriol. 1988 Sep;170(9):3847-54 PMID: 2842287
  24. Serological responses of patients with lepromatous and tuberculoid leprosy to 30-, 31-, and 32-kilodalton antigens of Mycobacterium tuberculosis.
    J Clin Microbiol. 1988 Oct;26(10):2200-2 PMID: 3141462
  25. H-2-linked control of in vitro gamma interferon production in response to a 32-kilodalton antigen (P32) of Mycobacterium bovis bacillus Calmette-Guérin.
    Infect Immun. 1988 Dec;56(12):3196-200 PMID: 3141285
  26. Humoral immune response in human tuberculosis: immunoglobulins G, A, and M directed against the purified P32 protein antigen of Mycobacterium bovis bacillus Calmette-Guérin.
    J Clin Microbiol. 1988 Sep;26(9):1714-9 PMID: 3053775
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.9.3205-3212.1991
Published
1991-09-00
Pages
3205-12
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258154
Subset
IM
Databases
GENBANK
S54032, S70201, S70204, X06392, X14442, X16435, X52899, X53033, X57105, X57229, X59240
External Links
PubMed source DOI: 10.1128/iai.59.9.3205-3212.1991 PMC Full Text View journal details
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