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PMID: 17475243 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Role of phosphatidylinositol-4-phosphate 5' kinase (ppk-1) in ovulation of Caenorhabditis elegans.

Experimental cell research ·Vol. 313 ·No. 11 ·2007-07-01 ·Pages 2465-75

Xu X, Guo H, Wycuff DL, Lee M

Abstract

During Caenorhabditis elegans ovulation, the somatic gonad integrates signals from germ cells and propels a mature oocyte into the spermatheca for fertilization. Previous work suggests that phosphoinositide signaling plays important roles in C. elegans fertility. To fully understand inositol-1,4,5-trisphosphate (IP(3)) signaling in ovulation, we have examined the function of phosphatidylinositol-4-phosphate 5' kinase (PIP5K) in C. elegans. Our results show that the C. elegans PIP5K homolog, ppk-1, is essential for ovulation in C. elegans; ppk-1 is mainly expressed in somatic gonad, and depletion of ppk-1 expression causes defective ovulation, reduced gonad sheath contractility, and sterility. Increased IP(3) signaling compensates for ppk-1 (RNAi)-induced sterility, suggesting that ppk-1 is linked to IP(3) signaling. These results demonstrate that ppk-1 plays an essential role in IP(3) signaling and cytoskeleton organization in somatic gonad.

MeSH Terms
Animals Animals, Genetically Modified Caenorhabditis elegans/genetics,physiology Caenorhabditis elegans Proteins/antagonists & inhibitors,genetics,physiology Cytoskeleton/metabolism,ultrastructure Female Inositol 1,4,5-Trisphosphate Receptors/metabolism Myosins/ultrastructure Ovulation/genetics Ovum/enzymology,ultrastructure Phosphotransferases (Alcohol Group Acceptor)/antagonists & inhibitors,genetics,physiology RNA Interference RNA, Small Interfering/pharmacology Signal Transduction
Chemicals
Caenorhabditis elegans Proteins Inositol 1,4,5-Trisphosphate Receptors RNA, Small Interfering Phosphotransferases (Alcohol Group Acceptor) 1-phosphatidylinositol-4-phosphate 5-kinase Myosins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Xu Xiaojian
Department of Biology, Baylor University, One Bear Place 97388, Waco, TX 76798, USA.
Guo Haisu
Wycuff Diane L
Lee Myeongwoo
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Article Info
Journal
Experimental cell research
Abbr.
Exp Cell Res
ISSN
0014-4827
DOI
10.1016/j.yexcr.2007.03.017
Published
2007-07-01
Epub
2007-00-24
Pages
2465-75
Language
English
Region
United States
NLM ID
0373226
PMCID
PMC1950138
Subset
IM
Grants
NIGMS NIH HHS · R15 GM077156 · United States
NIGMS NIH HHS · R15 GM077156-01 · United States
NIGMS NIH HHS · GM077156 · United States
External Links
PubMed source DOI: 10.1016/j.yexcr.2007.03.017 PMC Full Text View journal details
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