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PMID: 17526750 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical association of the NB-LRR resistance protein Rx with a Ran GTPase-activating protein is required for extreme resistance to Potato virus X.

The Plant cell ·Vol. 19 ·No. 5 ·2007-05-00 ·Pages 1682-94

Tameling WI, Baulcombe DC

Abstract

Nucleotide binding leucine-rich repeat (NB-LRR) proteins play an important role in plant and mammalian innate immunity. In plants, these resistance proteins recognize specific pathogen-derived effector proteins. Recognition subsequently triggers a rapid and efficient defense response often associated with the hypersensitive response and other poorly understood processes that suppress the pathogen. To investigate mechanisms associated with the activation of disease resistance responses, we investigated proteins binding to the potato (Solanum tuberosum) NB-LRR protein Rx that confers extreme resistance to Potato virus X (PVX) in potato and Nicotiana benthamiana. By affinity purification experiments, we identified an endogenous N. benthamiana Ran GTPase-Activating Protein2 (RanGAP2) as an Rx-associated protein in vivo. Further characterization confirmed the specificity of this interaction and showed that the association occurs through their N-terminal domains. By specific virus-induced gene silencing of RanGAP2 in N. benthamiana carrying Rx, we demonstrated that this interaction is required for extreme resistance to PVX and suggest that RanGAP2 is part of the Rx signaling complex. These results implicate RanGAP-mediated cellular mechanisms, including nucleocytoplasmic trafficking, in the activation of disease resistance.

MeSH Terms
GTPase-Activating Proteins/isolation & purification,metabolism Gene Silencing Immunity, Innate Leucine-Rich Repeat Proteins Molecular Sequence Data Plant Diseases/immunology Plant Proteins/isolation & purification,metabolism Potexvirus/physiology Protein Binding Protein Structure, Tertiary Proteins/isolation & purification,metabolism Solanum tuberosum/virology Tobacco/virology Virus Replication
Chemicals
GTPase-Activating Proteins Leucine-Rich Repeat Proteins Plant Proteins Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tameling Wladimir I L
Sainsbury Laboratory, John Ines Centre, Norwich NR4 7UH, United Kingdom.
Baulcombe David C
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.107.050880
Published
2007-05-00
Epub
2007-00-25
Pages
1682-94
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1913736
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · P18617 · United Kingdom
Databases
GENBANK
EF396237, EF396238
External Links
PubMed source DOI: 10.1105/tpc.107.050880 PMC Full Text View journal details
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