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PMID: 17646399 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast peroxisomes multiply by growth and division.

The Journal of cell biology ·Vol. 178 ·No. 3 ·2007-07-30 ·Pages 399-410

Motley AM, Hettema EH

Abstract

Peroxisomes can arise de novo from the endoplasmic reticulum (ER) via a maturation process. Peroxisomes can also multiply by fission. We have investigated how these modes of multiplication contribute to peroxisome numbers in Saccharomyces cerevisiae and the role of the dynamin-related proteins (Drps) in these processes. We have developed pulse-chase and mating assays to follow the fate of existing peroxisomes, de novo-formed peroxisomes, and ER-derived preperoxisomal structures. We find that in wild-type (WT) cells, peroxisomes multiply by fission and do not form de novo. A marker for the maturation pathway, Pex3-GFP, is delivered from the ER to existing peroxisomes. Strikingly, cells lacking peroxisomes as a result of a segregation defect do form peroxisomes de novo. This process is slower than peroxisome multiplication in WT cells and is Drp independent. In contrast, peroxisome fission is Drp dependent. Our results show that peroxisomes multiply by growth and division under our assay conditions. We conclude that the ER to peroxisome pathway functions to supply existing peroxisomes with essential membrane constituents.

MeSH Terms
Dynamins/metabolism Endoplasmic Reticulum/metabolism GTP Phosphohydrolases/genetics,metabolism GTP-Binding Proteins/genetics,metabolism Membrane Proteins/genetics,metabolism Mitochondrial Proteins Peroxins Peroxisomes/metabolism,ultrastructure Protein Transport Recombinant Fusion Proteins/genetics,metabolism Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Vesicular Transport Proteins
Chemicals
Membrane Proteins Mitochondrial Proteins PEX3 protein, S cerevisiae Peroxins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins GTP Phosphohydrolases GTP-Binding Proteins VPS1 protein, S cerevisiae DNM1 protein, S cerevisiae Dynamins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Motley Alison M
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, England, UK.
Hettema Ewald H
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200702167
Published
2007-07-30
Epub
2007-00-23
Pages
399-410
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2064844
Subset
IM
Grants
Wellcome Trust · United Kingdom
Wellcome Trust · 073257 · United Kingdom
External Links
PubMed source DOI: 10.1083/jcb.200702167 PMC Full Text View journal details
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