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PMID: 15928207 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Inp1p is a peroxisomal membrane protein required for peroxisome inheritance in Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 169 ·No. 5 ·2005-06-06 ·Pages 765-75

Fagarasanu M, Fagarasanu A, Tam YY, Aitchison JD, Rachubinski RA

Abstract

Cells have evolved molecular mechanisms for the efficient transmission of organelles during cell division. Little is known about how peroxisomes are inherited. Inp1p is a peripheral membrane protein of peroxisomes of Saccharomyces cerevisiae that affects both the morphology of peroxisomes and their partitioning during cell division. In vivo 4-dimensional video microscopy showed an inability of mother cells to retain a subset of peroxisomes in dividing cells lacking the INP1 gene, whereas cells overexpressing INP1 exhibited immobilized peroxisomes that failed to be partitioned to the bud. Overproduced Inp1p localized to both peroxisomes and the cell cortex, supporting an interaction of Inp1p with specific structures lining the cell periphery. The levels of Inp1p vary with the cell cycle. Inp1p binds Pex25p, Pex30p, and Vps1p, which have been implicated in controlling peroxisome division. Our findings are consistent with Inp1p acting as a factor that retains peroxisomes in cells and controls peroxisome division. Inp1p is the first peroxisomal protein directly implicated in peroxisome inheritance.

MeSH Terms
Cell Cycle/physiology Cell Division/physiology Extrachromosomal Inheritance/physiology GTP-Binding Proteins/metabolism Gene Expression Regulation, Fungal/genetics Intracellular Membranes/metabolism,ultrastructure Membrane Proteins/genetics,metabolism Peroxins Peroxisomes/genetics,metabolism,ultrastructure Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Saccharomyces cerevisiae Proteins/genetics,metabolism Vesicular Transport Proteins
Chemicals
INP1 protein, S cerevisiae Membrane Proteins PEX25 protein, S cerevisiae Peroxins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins GTP-Binding Proteins VPS1 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fagarasanu Monica
Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
Fagarasanu Andrei
Tam Yuen Yi C
Aitchison John D
Rachubinski Richard A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200503083
Published
2005-06-06
Epub
2005-00-31
Pages
765-75
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171609
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200503083 PMC Full Text View journal details
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