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PMID: 17660569 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A genomic screen in yeast reveals novel aspects of nonstop mRNA metabolism.

Genetics ·Vol. 177 ·No. 2 ·2007-10-00 ·Pages 773-84

Wilson MA, Meaux S, van Hoof A

Abstract

Nonstop mRNA decay, a specific mRNA surveillance pathway, rapidly degrades transcripts that lack in-frame stop codons. The cytoplasmic exosome, a complex of 3'-5' exoribonucleases involved in RNA degradation and processing events, degrades nonstop transcripts. To further understand how nonstop mRNAs are recognized and degraded, we performed a genomewide screen for nonessential genes that are required for nonstop mRNA decay. We identified 16 genes that affect the expression of two different nonstop reporters. Most of these genes affected the stability of a nonstop mRNA reporter. Additionally, three mutations that affected nonstop gene expression without stabilizing nonstop mRNA levels implicated the proteasome. This finding not only suggested that the proteasome may degrade proteins encoded by nonstop mRNAs, but also supported previous observations that rapid decay of nonstop mRNAs cannot fully explain the lack of the encoded proteins. Further, we show that the proteasome and Ski7p affected expression of nonstop reporter genes independently of each other. In addition, our results implicate inositol 1,3,4,5,6-pentakisphosphate as an inhibitor of nonstop mRNA decay.

MeSH Terms
Adaptor Proteins, Signal Transducing Codon, Terminator Exoribonucleases Genome, Fungal Genomics/methods Inositol Phosphates Mutation Proteasome Endopeptidase Complex RNA Stability/genetics RNA, Messenger/metabolism Saccharomyces cerevisiae Proteins/physiology
Chemicals
Adaptor Proteins, Signal Transducing Codon, Terminator Inositol Phosphates RNA, Messenger SKI7 protein, S cerevisiae Saccharomyces cerevisiae Proteins inositol-1,3,4,5,6-pentakisphosphate Exoribonucleases Proteasome Endopeptidase Complex
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wilson Marenda A
Department of Microbiology and Molecular Genetics, University of Texas Health Science Center, Houston, Texas 77030, USA.
Meaux Stacie
van Hoof Ambro
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.107.073205
Published
2007-10-00
Epub
2007-00-29
Pages
773-84
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC2034642
Subset
IM
Grants
NIGMS NIH HHS · R01 GM069900 · United States
NIGMS NIH HHS · GM069900 · United States
External Links
PubMed source DOI: 10.1534/genetics.107.073205 PMC Full Text View journal details
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